A step-by-step workflow for a PDCD1 knock-out experiment in T cells

This step-by-step tutorial video demonstrates the process of conducting a PDCD1 gene knock-out in T cells using the Neon™ NxT Electroporation System. See how to design the experiment using the Invitrogen™ TrueDesign™ Genome Editor software; prepare both cells and reagents; conduct post-transfection assessment; and validate the success of the PDCD1 knock-out for further experimentation. For additional support designing CRISPR gene editing experiments, visit thermofisher.com/geneediting or explore the other tutorial videos in this series:

• A step-by-step workflow for a knock-out experiment in an adherent cell line >>
• A step-by-step workflow for a knock-in experiment in an adherent cell line >>
• A step-by-step workflow for a knock-out experiment in iPSCs >>