New Methods to Improve Your Nucleic Acid and Protein Quantification

The latest technologies and methods for the molecular biology lab

21 Oct 2015
Sarah Thomas
Associate Editor

New technologies to improve your DNA, RNA and protein quantification

Quantification of nucleic acid and protein is very important to ensure the integrity of downstream applications. Here we present some of the latest technologies and methods added to SelectScience to help you ensure accurate quantification from the start of your experiment. Plus, discover the answers to some of the most asked photometry questions.

Discover the new Thermo Scientific™ NanoDrop™ microvolume UV-Vis spectrophotometer. The instrument incorporates new capabilities that make it even faster and easier to evaluate DNA, RNA and protein samples. Read more>>

Eppendorf´s R&D team answers the most frequently asked questions about photometry - a basic application in molecular biology labs - in a series of videos. Watch episode one>>

This application note evaluates how the accuracy of protein quantitation can be affected by fractionation at the protein level. Reproducible quantitation of cellular proteins is an important step in the analysis of cellular physiology. Protein-level fractionation in conjunction with tandem mass tag methods were used, using human gastric carcinoma cell line (MKN-45) as the example. Download method>>

Watch this SelectScience interview to learn how researchers at the Biocontrol and Natural Products (BANP) Group at Swansea University use BioTek Synergy H1 to efficiently perform a range of analyses, including protein quantification. Watch video>>

This note from Trinean describes a new cDrop™ application suited for the quantification (ng/μl) of DNA extracted from blood samples using the Promega ReliaPrep™ DNA isolation kit. Download method>>

The DeNovix DS-11 FX series spectrophotometer / fluorometer combines fluorescence and microvolume absorbance methods in a single, compact instrument. Rapidly quantify nucleic acid and protein samples over seven orders of magnitude, selecting from your choice of fluorescence assays, 1 µL absorbance or cuvettes. Read more>>

Bio-Rad Laboratories, Inc. recently announced the launch of ddPCR™ Supermix for Residual DNA Quantification. Designed for use with Bio-Rad’s Droplet Digital™ PCR Systems, this supermix provides a highly precise and sensitive method for directly measuring host cell DNA (HCD) that contaminates biopharmaceutical products. Read more>>

  • Download Exclusive Buying Guides

Download the free SelectScience UV/Vis Buying Guide and DNA and RNA Purification and Isolation Buying Guide to learn about important considerations when purchasing new products for molecular biology research.

Image copyright: Shutterstock hywards

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