ChIP-IT® FFPE Kit

Active Motif’s ChIP-IT FFPE assay is the first-of-its-kind to enable extraction and ChIP analysis of chromatin from FFPE samples for Next-generation sequencing, enabling researchers to uncover epigenetic variances in archived tissue.

The assay comes in two modules:

• ChIP-IT^® FFPE Chromatin Preparation Kit – extract high-quality ChIP-grade chromatin from preserved human, mouse or rat FFPE material. The kit has been successfully used to extract chromatin from FFPE blocks stored over 10 years under suboptimal conditions.
• ChIP-IT^® FFPE Kit – perform ChIP using the only ChIP kit available that can enrich for ChIP DNA using FFPE-extracted chromatin. The assay produces ChIP-enriched DNA in sufficient amounts and quality for analysis by qPCR or Next-generation sequencing.

ChIP-IT^® FFPE Features:

• Obtain quality chromatin using histological slides or tissue blocks as the starting material
• Sensitive enrichment of DNA from nanogram quantities of FFPE chromatin
• Optimized reagents reduce background levels caused by non-specific binding events
• Filtration based washes offer a faster, easier solution with better consistency than magnetic capture
• Highly robust procedure has been validated using FFPE chromatin from both normal and tumor samples with proven performance in both qPCR and ChIP-Seq analysis
• Controls are included in both the chromatin preparation and ChIP kits to help confirm results