CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag. CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows Formulation: CUTANA™ pAG-MNase is provided as a 20…
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Great result, reproducible data, reduced time and cost.
Perform CUT & RUN
I am an expert in CHIP-seq, and now I am an expert in CUT & RUN. Initially, I had to prepare everything and purify the protein pAG-MNase that consumed my time and increased my effort with less reproducibility. Since buying the CUTNA kit from EpiCypher, I have got very reliable data with the highest reproducibility. The kit contains all the reagents and enzymes with useful protocol to perform CUT & RUN experiments. I highly recommend this product for those who would like to perform CUT & RUN experiments.
Review Date: 12 Jun 2020 | EpiCypher
Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag.
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows Formulation: CUTANA™ pAG-MNase is provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50% glycerol.
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows Storage and Stability: Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows Application Notes: This product is sufficient to perform 50 CUT&RUN reactions. Recommended use: 2.5 μL of the supplied enzyme into a 50 μL CUT&RUN reaction (20X dilution). For detailed applications and uses of this product, please see Skene et al., 2018.
