DreamTaq Green PCR Master Mix
Thermo Scientific DreamTaq Green PCR Master Mix is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs.
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Very good results for pollen DNA Amplification!
DNA PCR Amplification of Pollen DNA for Metabarcoding
This product was really useful. I was able to amplify the DNA easily with very few steps. The online videos made it easy to follow and the fact that it already contains the tracking dye made it easy to then perform gel electrophoresis.
Review Date: 10 Jan 2023 | Thermo Fisher Scientific
Noise-free result
Polymerase Chain Reaction
The product quality is very good. Very sensitive to reaction. Noise-free determination.
Review Date: 27 Jul 2022 | Thermo Fisher Scientific
Good results, easy to use
Qualitative PCR
The mastermix is very, very easy to use and works very well for all the routine PCR primers that we use. Great value for a master mix and solid polymerase.
Review Date: 21 Jun 2021 | Thermo Fisher Scientific
Description
Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
Highlights
• Direct loading of PCR product on gel
• High yields and high sensitivity of PCR
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Robust amplification with minimal optimization
• Incorporates modified nucleotides, but does not incorporate dUTP
Applications
• Routine PCR amplification of DNA fragments up to 6 kb
• Genotyping
• High throughput PCR
