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FastNuclease™ Endonuclease Serratia Marcescens ELISA

ELISA for Quantitative Determination of Serratia endonuclease in Cell Culture and Biological Samples.

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Introduction:

Serratia marcescens nuclease originates from Gram negative bacteria S. marcescens and heads a family of homological non-specific nucleases that are widely spread in the world. Serratia nuclease is most studied one and it is capable to cleavage both RNA and DNA in either single or double stranded form.

Intended Use:

The Endonuclease Serratia marcescens ELISA kit developed for quantitative determination of endonuclease in samples from downstream processing where endonuclease is used as a process or purification aid. This ELISA is a sensitive and specific assay to detect and quantify residual endonuclease impurity in recombinant viral vector and vaccine preparations.

Principle:

The Endonuclease Serratia marcescens ELISA is a competitive immunoassay for the determination of Serratia endonuclease in sample.

About the Endonuclease S.marcascens assay:

  • Validated for leading endonuclease from various manufacturers
  • Comes with Concentrated Standards and precoated plates
  • Highly sensitive detection at competitive prices
  • Calibrator Range: 0 ng/ml - 256 ng/ml
  • Sensitivity: 0.1 ng/ml
  • Sample Type: Cell Culture Supernatant and other Biological Preparations
  • Shipping Condition: 2 - 8 Deg C
  • Detection Method: Colorimetric
  • Usage: For Research Use Only
  • Sensitive measurement of contaminants
  • Low intra-assay and inter-assay variability allows for streamlined testing High specific for the target analyte
  • Lot to Lot consistency.
  • Species Independent.
  • Priced better than competitors
  • Validated for various sample matrices

Krishgen kits have over 1000 citations, with the ELISA kits being used for publications in 15+ countries.

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