Ficoll-Paque PLUS and Ficoll-Paque PREMIUM
Ficoll-Paque PLUS Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes for in vitro studies. Low levels of endotoxin (< 0.12 EU/ml). Maintains viability and representative distribution of B and T lymphocytes. Ready to use sterile medium for isolation of human lymphocytes in high yield from peripheral blood. Ficoll-Paque PLUS is a ready to use, sterile me…
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A standard sterile medium for isolating human immune cells for in vitro studies.
PBMC isolation
Ready to use density gradient medium for high yield isolation of human, mouse, and rat low-density mononuclear cells from peripheral blood. It allows PBMCs to be isolated from whole blood upon centrifugation.
Review Date: 13 Dec 2022 | Cytiva
Efficient separation of granulocytes and PBMCs!
Separation of PBMCs from erythrocytes and granulocytes
The Ficoll-Paque gradient has been wonderful for separating PBMCs from granulocytes and red blood cells. We use a specific cell type that isn't as characterized as others and has some similarities to granulocytes, without the denseness, so using this Ficoll helps to make sure we separate as much of the granulocytes as possible from the cell type we want in the PBMCs. We place the Ficoll at the bottom of our tubes after the blood is already in, so it's a bit of an art to make sure the Ficoll stays separated from the blood, but once you get it, it separates wonderfully each time.
Review Date: 11 Dec 2019 | Cytiva
Ficoll-Paque PLUS
Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes for in vitro studies.
- Low levels of endotoxin (< 0.12 EU/ml).
- Maintains viability and representative distribution of B and T lymphocytes.
- Ready to use sterile medium for isolation of human lymphocytes in high yield from peripheral blood.
Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes.
Ficoll-Paque PREMIUM
Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.
- Cytotoxicity grade 0.
- Low levels of endotoxin (< 0.12 EU/ml) secured and tested.
- A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
- Manufactured under a Quality Management System certified to ISO 13485:2003.
- Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.
Applications
Ficoll-Paque PREMIUM
Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Ficoll-Paque PREMIUM 1.084
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Ficoll-Paque PREMIUM 1.073
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
