FLUOstar® Omega Plate Reader
The FLUOstar Omega is a multimode microplate reader perfect for life science research laboratories.
Colorimetric assays
Works like a charm and gives precise readings in a user friendly manner.
Review Date: 12 Sept 2024 | BMG LABTECH
Biochemistry
We got our Fluostar Omega in 2010. It has been in frequent regular use since then and was moved between three institutions. It never failed us. Last year we got it serviced for the first time because it made a rattling noise. Customer service was perfect. It is running smoothly again and I have hopes that it will survive beyond my retirement.
Review Date: 24 Jul 2024 | BMG LABTECH
Plate reading
We purchased this system more than 10 years ago and all its functions are regularly used. We never experienced any issue with this machine. We only did the regular maintenance and it never failed us so far. Although it is frequently used by inexperienced users (students internships) and people from other labs for single measurements. It is very user-friendly, easy to personalize for each user and always achieves excellent measurement if correctly used. The precision of this machine taught many students to put more effort in the precision of their cell culture pipetting.
Review Date: 24 Jul 2024 | BMG LABTECH
Bacterial growth and fluorescent reads
The machine is so easy to use. The software is easy to learn and I appreciate being able to put MARS on my own laptop and manage my runs. Sylvia was amazing doing the set up and initial tutorials and always helpful for troubleshooting.
Review Date: 12 Jul 2023 | BMG LABTECH
Enzyme activities, ELISA, Biomarkers
Easy to use for data calculation but needs for technical assistant to fill the number of gain in fluorescence measurement.
Review Date: 6 Jun 2023 | BMG LABTECH
Cellular modeling of Parkinson's disease
Reader is user friendly with relatively intuitive software. One of only a couple readers that will run proper long term shaking prion-like assays.
Review Date: 20 Apr 2023 | BMG LABTECH
Microbiology, plant science
High quality and reproducible results. Time-based assay with slow kinetics option saved so much time and hassle in the lab. Easy to use. Excellent customer service by Sylvia! Thank you.
Review Date: 30 Nov 2022 | BMG LABTECH
Analyse the viability, toxicity, and metabolic activity of cells
I have done some experiments for cell analysis (resazurin, prestoblue, Hoechst, acridine orange, …) and protein (Bradford). I achieve high-quality and reproducible results in a short time. The software for working with the machine is simple and user-friendly. The software for processing the data is also good. I am satisfied with working with this machine.
Review Date: 25 Nov 2022 | BMG LABTECH
Ca2+ Fluorimetry
Our group has been using Fluostars for more than a decade now. The instruments are very reliable, are easy to use and intuitive to operate in a basic research environment.
Review Date: 22 Nov 2022 | BMG LABTECH
Analysing presence of antibodies that bind to antigen of inyerest ELISA
It is easy to use and I obtain high quality results
Review Date: 16 Nov 2022 | BMG LABTECH
The FLUOstar Omega is a multimode microplate reader perfect for life science research laboratories.
Capable of measuring all of the leading microplate based assays, one of its most unique features is its CCD-based spectrometer. This proprietary spectrometer can capture an entire UV/Vis absorbance spectra (220 to 1000 nm) at read times faster than 1 second per well. This ultra-fast, full spectrum analysis allows for assays not possible on other microplate readers.
Voted Select Science’s Best Drug Discovery Product of 2010, the FLUOstar Omega multi-detection microplate reader offers the following detection modes:
- Fluorescence Intensity - including FRET
- Time-Resolved Fluorescence - including DELFIA®
- TR-FRET- including HTRF® and LanthaScreenTM
- AlphaScreen®/AlphaLISA®
- High-Performance Luminescence (flash and glow) - including BRET, DLReady™
- Ultra-fast, UV/Vis Absorbance
The FLUOstar Omega multimode microplate reader provides users with unmatched flexibility and sensitivity to perform any assay needed in all microplate formats up to 1536-well.
ACU - Atmospheric Control Unit for Cell-based Assays
For cell-based assays, the FLUOstar Omega has a dual gas controller that independently regulates both CO2 and O2. With the microprocessor controlled ACU, oxygen levels can be monitored between 1% and 19%, and CO2 levels can be regulated between 0% and 20%.
Low Volume Measurements with the LVis Plate
For low volume measurements in assays like DNA, RNA, or protein concentration determination, the FLUOstar Omega can use BMG LABTECH’s LVis Plate. The LVis plate has sixteen microdrop wells in which two microliters of sample can be measured.
Upgrades
The Omega microplate reader series has a modular design concept. This enables the FLUOstar Omega to be fully upgradeable to the powerful POLARstar Omega, which can perform fluorescence polarization using Simultaneous Dual Emission detection. In addition, up two reagent injectors can be added for kinetic assays.
Software
The FLUOstar Omega comes with BMG LABTECH's license-free Omega Reader Control software and MARS Data Analysis Software. This highly intuitive software package provides limitless possibilities for test set up, but also offers easy one-click App buttons for the most common applications.
For cell-based assays, the FLUOstar Omega has a dual gas controller that independently regulates both CO2 and O2. With the microprocessor controlled ACU, oxygen levels can be monitored between 1% and 19%, and CO2 levels can be regulated between 0% and 20%.
Applications:
- Calcium measurements (e.g. Ca2+ flux with Fura-2, Indo-1, FLUO-3/4)
- Enzyme activity
- FRET/BRET assays
- Cell toxicity, proliferation, and viability
- Cell migration and invasion
- Stem, mammalian, and microbial cells
- Protein and nucleic acid quantitation (e.g. Bradford, Lowry, RNA, DNA, 260/280 nm)
- ATP or ADP quantification
- Immunoassays, ELISA
- Luciferase, dual luciferase, Renilla, Firefly, Cypridina, Gaussia
- ORAC and ABEL antioxidant assays
- Fluorescent proteins like GFP, CFP/YFP FRET
- TR-FRET assays, including HTRF® and LanthaScreenTM
- AlphaScreen® and AlphaLISA Assays
Accessories
- Atmospheric Control Unit (ACU): provides the ideal environment for cell-based assays by regulating O2 and CO2 gas levels in the microplate reader
- Filters and Optic Modules: standard filters from 240 - 850 nm, filters and Optic Modules optimised for specific applications, and bespoke solutions are available
- Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
- LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
- Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purposes