GST and 6HIS tag check kits
An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…
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An easy way to assess tag accessibility
The production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind directly to XL665-conjugated GST or 6HIS peptide.
A signal decrease proportional to the amount of tagged protein to be tested is the sign of accessibility of the tagged moiety for the corresponding HTRF® toolbox reagents (anti-GST or anti-6HIS).
Each kit yields 1,000 test determinations using a 20 µL final volume. Each kit contains lyophilized anti-GST or anti 6HIS Cryptate conjugates, and GST-XL665 or 6HIS-XL665 conjugates.
It also includes a maximum calibrator for the constitution of the calibration curve (GST check kit) or a kit control (for 6HIS check kit). Reconstitution buffer and diluent are supplied for both kits.
