Invitrogen™ LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit
The LIVE/DEAD Fixable Blue Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…
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The LIVE/DEAD Fixable Blue Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with compromised membranes, whereas this reactivity is restricted to amines on the cell surface in viable cells. The fluorescence difference between dead and live cells is greater than 50-fold. The staining pattern is preserved following formaldehyde fixation.
Features
- Stable—dyes are freeze dried in separate vials to maintain stability
- Robust—staining pattern is the same before and after fixation
- Bright signal—allows for easy distinction between live/dead cells in single channel
The LIVE/DEAD Fixable Blue Stain is excited with a UV laser with an excitation maximum of approximately 350 nm, and an emission of approximately 450 nm. The use of a UV laser frees up a flow cytometry channel for other reagents and results in no overlap issues with common dyes, eliminating the need for compensation assays. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments. This kit has been optimized and validated for use with a UV laser flow cytometer.
