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iScript™ Reverse Transcription Supermix, 25 x 20 µl rxns, 100 µl

Bio-Rad1708840

25 x 20 µl reactions, 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

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Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.

Key Features and Benefits

  • Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
  • Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
  • Amplify cDNA for genes with low expression levels
  • Fast 26-minute 1-tube cDNA synthesis protocol
  • 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination


Related Products

  • cDNA Synthesis Kits
  • Real-Time PCR Supermixes and Kits
  • PrimePCR™ PCR Primers & Assays

Related Categories

  • PCR and qPCR Reagents
  • Real-Time PCR Detection Systems
  • PCR Plastic Consumables
  • Instrument Validation Tools and Services

Online Resources

  • qPCR/Real-Time PCR
  • Genomics

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