Products & ReviewLife Sciences
iScript™ Reverse Transcription Supermix, 25 x 20 µl rxns, 100 µl
Bio-Rad1708840
25 x 20 µl reactions, 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix
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Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.
Key Features and Benefits
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
- Amplify cDNA for genes with low expression levels
- Fast 26-minute 1-tube cDNA synthesis protocol
- 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
Related Products
- cDNA Synthesis Kits
- Real-Time PCR Supermixes and Kits
- PrimePCR™ PCR Primers & Assays
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Online Resources
- qPCR/Real-Time PCR
- Genomics
