iST-NHS Sample Preparation Kit
Easy to use all-in-one sample preparation solution for LC-MS-based proteomics. Based on in-StageTip (iST) technology and compatible to various different sample types as well as chemical labelling.
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Sample preparation is one of the most critical steps in MS-based proteomic workflows, but the variability of the starting materials (cell/tissue type and protein levels) can be challenging. There is a need for easy-to-use and automatable workflows, along with all-in-one sample preparation solutions, to improve the results' consistency and reliability.
The iST technology works with sample preparation from plant or mammalian cells and tissues, and with human biological fluids (e.g. plasma). iST-NHS is compatible with chemical labeling (iTRAQ and TMT). An increased peptide and protein identification is provided in runs of 8 to 96 samples in a working range of 1-100 µg protein.
Unlike other sample preparation workflows the iST sample preparation allows Lysis denatutaion, reduction and alkylation in one single step and thus only needs < 3 hours to get from protein to the purified peptide.
