KAPA Long Range PCR Kits
The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the fi…
Amplify long targets with high sensitivity
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The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability.
This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.
Features:
Amplify long targets with high sensitivity
- Amplification of targets with high sensitivity and specificity up to 15 kb
- High yields with low input DNA
Outperforms with complex targets
- Exhibits high yield, greater sensitivity and specificity where competitor long-range systems fail
Applications:
- Amplification of long targets and/or low amounts
- Standard short- and mid-range PCR
- T-A cloning
- Targeted sample enrichment for next-generation DNA sequencing
