Products & ReviewLife Sciences

KAPA RNA Library Preparation Kits for Illumina

Kapa Biosystems, Inc.Available: Worldwide

KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved…

Kapa Biosystems, Inc.

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Ease of Use
After Sales Service
Value for Money
Write your own review

KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA.

KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved coverage of GC-rich and low-abundance transcripts. Kits contain KAPA HiFi for high-efficiency and low bias library amplification, as well as KAPA mRNA Capture Beads and a streamlined, “with-bead” protocol.

KAPA Stranded RNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, but do not contain the KAPA mRNA Capture Beads. Kits can be used to prepare libraries from 10-400 ng of either poly(A)-selected, ribosomally-depleted, or total RNA.

Features:

Uncover challenging transcripts

  • Improved coverage of GC-rich transcripts
  • Enhanced identification of exonic regions

Detect low-abundance transcripts

  • Enables identification of transcripts missed by competitor kits, even with high input
  • High uniformity across varying amounts of sample input

Identify more genes

  • Higher percentage of uniquely mapped reads compared to Illumina TruSeq™ Stranded mRNA Sample Prep Kits
  • Lower duplication rates yield better coverage

Maintain high coverage uniformity

  • Minimal 5′–3′ bias across transcripts
  • More uniform distribution of reads over each transcript

Applications:

  • Gene expression
  • Single nucleotide variation (SNV) discovery
  • Post-transcriptional SNVs
  • Fusion gene identification
  • Targeted transcriptome
  • Whole transcriptome

Product Overview

Links