KAPA RNA Library Preparation Kits for Illumina
KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved…
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KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA.
KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved coverage of GC-rich and low-abundance transcripts. Kits contain KAPA HiFi for high-efficiency and low bias library amplification, as well as KAPA mRNA Capture Beads and a streamlined, “with-bead” protocol.
KAPA Stranded RNA-Seq Kits include all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, but do not contain the KAPA mRNA Capture Beads. Kits can be used to prepare libraries from 10-400 ng of either poly(A)-selected, ribosomally-depleted, or total RNA.
Features:
Uncover challenging transcripts
- Improved coverage of GC-rich transcripts
- Enhanced identification of exonic regions
Detect low-abundance transcripts
- Enables identification of transcripts missed by competitor kits, even with high input
- High uniformity across varying amounts of sample input
Identify more genes
- Higher percentage of uniquely mapped reads compared to Illumina TruSeq™ Stranded mRNA Sample Prep Kits
- Lower duplication rates yield better coverage
Maintain high coverage uniformity
- Minimal 5′–3′ bias across transcripts
- More uniform distribution of reads over each transcript
Applications:
- Gene expression
- Single nucleotide variation (SNV) discovery
- Post-transcriptional SNVs
- Fusion gene identification
- Targeted transcriptome
- Whole transcriptome