Products & ReviewLife Sciences

KASP™ Genotyping Reagents

LGC Biosearch TechnologiesAvailable: Worldwide

Unmatched quality, reliability and credibility have established KASP as the foundational SNP genotyping chemistry for the Agrigenomics industry. 

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Average Rating 4.5

|31Scientists have reviewed this product

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Great results, relatively economical prices

 

Average Rating 4.0

Application Area:

Genotyping of sunflower

The need for high-quality DNA extraction is not an advantage. The manufacturer wants to be a part of our papers, If they provided the products and the services for free, this demand could be reasonable. Good kit, easy to apply. Not using gel electrophoresis is a huge advantage.

Review Date: 29 Jun 2022 | LGC Biosearch Technologies

Easy Chemistry

 

Average Rating 4.3

Application Area:

SNP Marker Testing for Plant Breeding

Works great! Easy to use.

Review Date: 29 Jun 2022 | LGC Biosearch Technologies

Reproducible and very high quality results.

 

Average Rating 4.7

Application Area:

KASP analysis for plant and animal beeding.

Magic and stable enzyme and primers that are used increasingly by the majority of SNP Genotyping scientists.

Review Date: 23 Jun 2022 | LGC Biosearch Technologies

It was a good experience. WIll use LGC again in future.

 

Average Rating 5.0

Application Area:

Genotyping to validate SNPs identified earlier

Good quality results. Initially there were some misunderstanding in what LGC understood about my requirement although my email was very clear. Mistakes were rectified immediately and I got a few more SNPs genotyped as a good will gesture from LGC. In all and all it was very easy to work with LGC.

Review Date: 1 Nov 2017 | LGC Biosearch Technologies

Great results

 

Average Rating 5.0

Application Area:

Biotech in research of corn seeds in Brazil

The best product we are working in reasearch of corn seeds. We are obtain great results.

Review Date: 1 Nov 2017 | LGC Biosearch Technologies

Excellent - a quick and reliable way to genotype.

 

Average Rating 5.0

Application Area:

Genotyping

We originally started using KASP genotyping assays for SNP genotyping but have found it so easy to use and cost-effective that we have now switched to genotyping all our insertion and deletion mutations this way as well. We have had bespoke assays designed and the support has been great.

Review Date: 8 Aug 2017 | LGC Biosearch Technologies

Great results

 

Average Rating 4.7

Application Area:

Population genetics of marine mussels

I obtained very nice results with a low rate of missing data and reproducible genotypes. When compared with BeadXpress genotyping the KASP chemistry provided much nicer results.

Review Date: 7 Aug 2017 | LGC Biosearch Technologies

gread assay and good results

 

Average Rating 5.0

Application Area:

create KASP Assays

The order steps of KASP Assay are not so easy to handle but after this ist so great. The service with the emails of the state of order and shipment. is very good.

Review Date: 2 Aug 2017 | LGC Biosearch Technologies

Excellent data supply

 

Average Rating 4.0

Application Area:

Genotyping facility

The test result is handy and can be viewed in good excell format.

Review Date: 2 Aug 2017 | LGC Biosearch Technologies

Thanks for your patient explanations to us economists!

 

Average Rating 4.7

Application Area:

Analyze authenticity of maize seed samples collected in Uganda

The staff at LGC have been extremely kind and helpful! They have also been quite patient in dealing with economists who know very little about genotyping, and have explained processes clearly and patiently. They have been a pleasure to work with so far!

Review Date: 1 Aug 2017 | LGC Biosearch Technologies

The patented KASP™ genotyping method is a homogeneous, FRET-based end-point PCR assay that enables accurate bi-allelic discrimination of known SNPs and InDels.

Unlike other PCR-based genotyping assays, KASP requires no labelling of the target-specific primers or probes, giving it a clear cost advantage. It also allows greater flexibility for assay design, which translates into a higher overall success rate and the unique ability to genotype large/mega InDels. For over 20 years, the KASP assay has been cited in over 2,500 peer-reviewed publications, developed into tens of thousands of pre-validated assays across many commercially important crops, and used to develop over 200,000 SNP genotyping assays in more than 75 organisms, including higher ploidy plant species.

Whether purchased as a product for use in your laboratory, or as a genotyping service at our state of the art genotyping laboratories in Europe and North America, KASP genotyping delivers accurate results and significant cost savings so customers can get the most out of their research budget. KASP genotyping is based on an open format PCR workflow. There is no need to buy new expensive equipment to be able to run KASP in your own laboratory; completed KASP reactions can be read on most FRET-capable plate readers and qPCR instruments.

Features:

  • Accuracy: >99.8% based on independent assessment
  • Industry leading SNP & InDel assay conversion rate (>90%)
  • Flexible primer design which increases the rate of successful assay development
  • Supports low, medium and high-throughput studies and individual repeat assays
  • Compatibility with a wide range of liquid handling systems and thermal cyclers, signal can be read on most FRET-capable plate readers and qPCR instruments
  • Breakthrough cost savings

Applications:

  • Many applications in plant, animal, human and microbial research
  • Plant Marker-assisted selection for crop improvement (including MARS and MABC)
  • Fingerprinting / parental screening
  • SNP validation
  • Field trial genotyping
  • Animal Marker-assisted selection for livestock breeding
  • Speciation
  • Sample provenance and quality control
  • Mapping traits within disease models and breeding populations (e.g. zebrafish, mouse, rat)
  • Speed congenics
  • Human Genetic variant analysis for human disease research
  • Cohort studies
  • Disease SNP replication/validation studies

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