miRNA Target IP Kit
Designed to identify the physical interactions of a miRNA with endogenous mRNA transcripts
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The miRNA Target IP Kit utilizes protein G-coupled magnetic beads and a pan-Ago antibody that recognizes Ago 1, Ago 2 and Ago 3 proteins to identify the physical interactions of miRNAs with endogenous mRNA transcripts.
The kit supplies sufficient reagents to perform 10 miRNA Target IP reactions using the included Ago1/2/3 Antibody and 10 negative control IP reactions using the included Negative Control IgG. Following their immunoprecipitation as part of the RISC complex, the mRNA targets can be purified and then amplified by qRT-PCR using gene-specific primers.
By comparing cells transfected with or without a miRNA mimic, one can identify the targets of a particular miRNA based on over-expression of that miRNA.
