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mTeSR™ Plus

Stabilized feeder-free maintenance medium for human ES and iPS cells

STEMCELL Technologies Inc.

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mTeSR™ Plus is a serum-free, stabilized cell culture medium for the feeder-free maintenance and expansion of human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is based on the mTeSR™1 formulation, the most widely published feeder-free cell culture medium for human ES and iPS cells.

To enhance cell-quality attributes during restricted feeds, critical medium components have been stabilized, including FGF2, and medium pH is more consistent. As a result, mTeSR™ Plus allows for greater cell expansion rates with daily feeding, while also maintaining cell quality during restricted feeding schedules.

mTeSR™ Plus is compatible with a variety of culture matrices, including Corning® Matrigel® hESC-Qualified Matrix (Catalog #07181) and Vitronectin XF™ (Catalog #07180, a matrix developed and manufactured by Nucleus Biologics).

Each lot of mTeSR™ Plus 5X Supplement is used to prepare complete mTeSR™ Plus medium and then performance-tested in a culture assay using human pluripotent stem cells (hPSCs).

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