Premix Ex Taq™ (Probe qPCR)

Premix Ex Taq (Probe qPCR) Master Mix

Takara Bio Premix Ex Taq (Probe qPCR) is a 2X master mix for real-time PCR (qPCR) using probe-based qPCR or 5' nuclease assays. This 2X master mix includes TaKaRa Ex Taq HS, a hot start PCR enzyme in combination with anti-Taq antibody, in a qPCR optimized buffer. TaKaRa Ex Taq HS inhibits non-specific amplification while enabling high efficiency amplification and detection sensitivity during real-time PCR analyses. Additionally, Tli RNase H, a heat-resistant RNase enzyme, is included in the real time PCR premix in order to minimize PCR inhibition due to the presence of residual mRNA in the input cDNA. This master mix is ideal for high-speed PCR, allows accurate target quantification and detection over a broad dynamic range, and enables highly reproducible and reliable real-time PCR analyses.

Two tubes of ROX Reference Dye are supplied separately with Cat. #RR390A/B and RR390L/W. The ROX Dye serves as a convenient internal reference standard when normalizing signals resulting from non-PCR fluorescence fluctuations that occur between wells or over time in different instruments.

Cat.# RR390L/W are larger package sizes (5 mL and 25 mL (5 x 5 mL), respectively) of Premix Ex Taq (Probe qPCR). Each of these items provides convenient larger packaging and is supplied with a single, separate vial of ROX Reference Dye.

Cat.# RR39LR/WR are larger package sizes (5 mL and 25 mL (5 x 5 mL), respectively) that already contain ROX Reference Dye in the Real Time PCR Premix.

Features:

• Rapid and accurate product detection
• Quantitative gene expression analysis using real-time PCR (qPCR)
• 2X master mix enables easy pipetting
• Optimized buffer increases amplification efficiency and highly sensitive probe-based qPCR assays and 5' nuclease assays
• Included Tli RNase H minimizes PCR inhibition by removing residual mRNA in input cDNA

Applications:

• Real Time PCR (qPCR) quantitation of DNA using methods such as probe-based qPCR assays and 5' nuclease assays