PrimeSTAR® Max DNA Polymerase
PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…
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Outstanding and Robust DNA polymerase for all kinds of DNA amplification needs!
Polymerase chain reaction
One of the best DNA polymerase for difficult DNA amplification. It produces even long amplification with high fidelity. I can recommend it for best results.
Review Date: 16 Apr 2018 | Takara Bio
PCR
Takara's Primestar polymerase is fantastic. High yields, no errors, fast annealing. My lab uses it for site directed mutagenesis. Gives great yields and lots of correctly mutated colonies with high efficiency. May cost a little more than other enzymes, but we simply do smaller reaction volumes and that makes it more affordable.
Review Date: 3 Jun 2013 | Takara Bio
PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR.
Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension, greatly reducing the time required for annealing and extension steps. As a result, PrimeSTAR Max DNA Polymerase can be used for exceptionally fast PCR. Since PrimeSTAR Max DNA Polymerase is configured as a premix, it allows rapid preparation of reactions and is useful for high-throughput applications. For highly accurate amplification during cloning and expression studies, structural or evolutionary analyses, PrimeSTAR Max DNA Polymerase is the high fidelity polymerase of choice.
PrimeSTAR Max DNA Polymerase is also suitable for reactions that occur in the presence of excessive nucleic acid. Extraneous DNA ordinarily inhibits template amplification when using conventional polymerases because the amount of effective polymerase is limited by nonspecific binding. The superior processivity of PrimeSTAR Max DNA Polymerase prevents inhibition by excessive nucleic acid, resulting in a much higher success rate for PCR with minimal required optimization. Furthermore, the antibody-mediated hot start formulation of the polymerase prevents false initiation events from occurring during the reaction assembly.
Features:
- Highest fidelity of any commercially available DNA polymerase
- Fastest extension speed means less time required for PCR cycles
- Convenient 2X premix including buffer and dNTPs, allowing reaction assembly in less time
- Antibody-mediated hot-start formulation
- Proven performance as reported in peer-reviewed literature
Applications:
- Fast PCR for high throughput studies
- Cloning and expression studies
- Structure/function studies
- Evolutionary studies (e.g., SNP analyses)
