PrismA ELISA kit
The PrismA ELISA kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA ™ chromatography resin and Fibro ™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals.
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The PrismA ELISA kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA™ chromatography resin and Fibro™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals. Purification of mAbs is routinely performed using protein A based affinity chromatography, which provides high capacity and consistent purification performance. When using protein A based purification techniques, it is a regulatory requirement to measure leached protein A ligand in the generated downstream material. For the most accurate results, it is important that manufacturers use a resin-matched standard to calibrate their assay.
Key benefits
- Matched PrismA protein A ligand included in the kit removes the need to source the standard separately and create a custom protocol
- Parts per billion sensitivity minimizes the risk of undetected residual PrismA ligand
- High IgG tolerance simplifies sample preparation
- Consistently low intra- and inter-plate variability ensures reproducible data
- All-in-one kit containing all reagents needed to perform the assay
