PrismA ELISA kit

The PrismA ELISA kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA^™ chromatography resin and Fibro^™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals. Purification of mAbs is routinely performed using protein A based affinity chromatography, which provides high capacity and consistent purification performance. When using protein A based purification techniques, it is a regulatory requirement to measure leached protein A ligand in the generated downstream material. For the most accurate results, it is important that manufacturers use a resin-matched standard to calibrate their assay.

Key benefits

• Matched PrismA protein A ligand included in the kit removes the need to source the standard separately and create a custom protocol
• Parts per billion sensitivity minimizes the risk of undetected residual PrismA ligand
• High IgG tolerance simplifies sample preparation
• Consistently low intra- and inter-plate variability ensures reproducible data
• All-in-one kit containing all reagents needed to perform the assay