qScript cDNA Synthesis Kit
Economical 2-component kit ideally suited for high throughput gene-expression studies
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Good quality, cheap product that delivers.
Create cDNA from mRNA
I really love this cDNA synthesis kit. I've been using it for years now in two separate labs and all the students I talked to request that I purchase it again for their experiments. It ships with three components: a buffer, reverse transcriptase enzyme mix and some water. All you do is mix up to 1ug of RNA with the buffer and enzyme mix (I use my own water), incubate at 42c then 37c then 65c for inactivation, and tour done. Quick and easy for qPCR or just some RT PCR. It's also really cheap.
Review Date: 14 Apr 2023 | Quantabio
The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. This economical, highly stable stabilize, 2-component reagent system has been rigorously optimized to ensure sensitive and linear RNA detection with a wide-range of input RNA and relative abundance. Reagent performance is unaffected by repetitive freeze/thaw cycling (>20X), conferring greater ease-of-use and assay performance consistency. Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5' and 3'-sequences for accurate gene expression quantification. The resulting cDNA product is directly compatible with all qPCR chemistries or conventional end-point RT-PCR of amplified fragments ≤1 kb in length.
