Scherzo SM-C18 Column
An ODS column with Anion and Cation Exchange. Weak ionic ligands adequately bonded to this material optimized for the separation of basic/acidic compounds at neutral pH.
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The answer to (almost) all your retention problems!
Analysis of mixtures containing neutral, positively and negatively charged compounds
This column has been the answer to most of my retention problems! It combines four retention modes [normal-phase, reverse phase, anion-exchange and cation exchange, all in one!]. I have used it for numerous complex mixtures and got great resolution without requiring the use of ion-pairing reagents and with very reproducible results! It comes in several lengths and diameters which allows you to transition seamlessly from analytical to prep scale.
Review Date: 12 Feb 2020 | Imtakt
Give it a try!
Analysis of oligonucleotides and free nucleotides
I confess, I was a little skeptical about trying this column since it seemed to offer multiple separation modes (ion-exchange, reverse-phase, ion-pairing, and HILIC) all-in-one! I was pleasantly surprised to see not just how effective it was separating all my analytes, but also how sturdy and durable it turned out to be. I have been using it almost daily, for several months, with crude hydrolysates and I am pleased to report that so far, the efficiency and resolution have not been affected at all, despite the fact that, as I said, most of my test samples are less than pristine. It is available in a variety of packings, in different lengths and diameters, including semi-prep and the prices are in line with most analytical columns.
Review Date: 14 Jan 2020 | Imtakt
The Scherzo family columns can operate without adding an ion-pairing reagent which is required for conventional ODS columns. In addition, both anionic and cationic compounds are retained on this column. The hydrophobicity of the Scherzo family is similar to that of a conventional ODS colum, so analysis of a compound in combination with a Unison UK-C18 column (conventional ODS phase) will be very effective for method development.
The SM-C18 consists of not only ODS ligands, but also both weak anion ligands and cation ligands which is useful for a wide range of ionic compounds such as salts, vitamins, acidic/ basic compounds, alkaloids etc. It also provides reversed-phase mode, both ion exchange modes, and normal phase mode. Scherzo C18 columns are designed by using the same silica and ODS ligand density as a Unison UK-C18 column. SM-C18 may be effective in changing selectivity when it is difficult to separate impurities on an ODS column.
- Polar and non-polar retention in Reversed Phase conditions
- Multi-level retention without ion-pairing reagents
- Compatible with LC-MS
- Unique selectivity of HPLC column
- Wide range of method development possibilities
- Can simultaneous optimize organic, buffer and pH gradients
- Anion Exchange + Cation Exchange + Reversed Phase + Normal Phase
- Weak ionic ligands adequately bonded to this material optimized for the separation of basic/acidic compounds at neutral pH.
