Second Strand cDNA Synthesis Kit
Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure. Features of…
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Great product!
Allows easy cDNA to be synthesized from RNA
Highly effective and easy to use; the cDNA kit was easy to used and allowed us to quickly synthesized cDNA for other experiments. I also liked how the protocol for the kit was available online and it was easy to read and use.
Review Date: 11 Aug 2022 | Thermo Fisher Scientific
I can save a lot of time with great quality results.
Synthesis of cDNA using harvested RNA from virally infected cells
It is a great product. In particular, the quality of cDNA concentration. I'm happy I could get A260/280 concentration ~1.8 and good concentration of the synthesized cDNA.
Review Date: 24 Jul 2020 | Thermo Fisher Scientific
Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents
Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
