Sequencing Reaction Clean-Up Kit
For the rapid purification of sequencing extension products from reaction mixes
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DNA sequencing (Sanger sequencing)
The kit was very easy to use for cleanup of Sanger sequencing products prior to run on the ABI 3130xl genetic analyzer. After sequencing PCR, the PCR reaction as mixed with binding buffer and applied on the cleanup column. It was followed by 2 washes. The product was eluted as suggested by kit. Quick and easy to use protocol. No shaking needed for a long time. Cleanup finished within 5 minutes. As easy a clumn DNA purification. The sequencing chromatograms were clean.
Review Date: 9 Jun 2019 | Norgen Biotek Corp.
For the rapid purification of sequencing extension products from reaction mixes
- Purify sequencing extension products from dye terminators, primers and other contaminants
- Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
- High recovery
- Fast and efficient spin column format
- Also available in 96 well format for high throughput