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T4 DNA Ligase

Thermo Scientific T4 DNA Ligase is a single polypeptide. It is commonly used to join DNA molecules to each other. T4 DNA ligase catalyzes the formation of a phosphodiester linkage between DNA chains by condensing the 5'-phosphoryl group of one DNA chain with the adjacent 3'-hydroxyl group of the other DNA chain. T4 DNA ligase will ligate blunt and homologous cohesive ends of double-stranded DNA. It is the only DNA ligase that…

Thermo Fisher Scientific

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Average Rating 4.1

|3Scientists have reviewed this product

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We've tried other brands but this works best

 

Average Rating 4.3

Application Area:

Determination of breast cancer genetic variants in blood samples

It is really effective, but it comes in very small volume/units, so it finishes promptly.

Review Date: 23 Feb 2024 | Thermo Fisher Scientific

Good robust results

 

Average Rating 4.0

Application Area:

Gene cloning

It’s really great product, works very efficiently. I use it for cloning.

Review Date: 15 May 2017 | Thermo Fisher Scientific

Average Rating 4.0

Application Area:

Developing SNP markers in tilapia species using double digest RAD sequencing

T4 DNA ligase is very effective enzyme to joining DNA fragment produced from cutting site of enzyme restriction SbfI and SphI to a barcode sequences. The barcode can be perfectly recalled in the analysis after sequencing the DNA library.

Review Date: 8 Jan 2015 | Thermo Fisher Scientific

Thermo Scientific T4 DNA Ligase is a single polypeptide. It is commonly used to join DNA molecules to each other. T4 DNA ligase catalyzes the formation of a phosphodiester linkage between DNA chains by condensing the 5'-phosphoryl group of one DNA chain with the adjacent 3'-hydroxyl group of the other DNA chain. T4 DNA ligase will ligate blunt and homologous cohesive ends of double-stranded DNA. It is the only DNA ligase that will efficiently ligate blunt ends in the absence of macromolecular exclusion molecules. T4 DNA Ligase can repair nicks in duplex DNA, RNA or DNA/RNA hybrids. It can also join RNA single strands via bridging oligonucleotide adaptors.

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