Whole IgG Affinity-Purified Secondary Antibodies
Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography.
Quality product..
Provides quality secondary antibodies that are highly affordable with more conjugation options than any other vendor I'm familiar with. It has great quality and it never disappoint. Great tech support as well
Review Date: 31 Jul 2022 | Jackson ImmunoResearch Laboratories Inc.
Immunology
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins.
Review Date: 31 Mar 2021 | Jackson ImmunoResearch Laboratories Inc.
Immunofluorescence
Jackson's secondary antibodies and antibody fragments are always of the highest quality and work perfectly the first time, every time. I've used them for over 30 years now and have never been disappointed.
Review Date: 1 Jul 2020 | Jackson ImmunoResearch Laboratories Inc.
Immunohistochemistry/IF
Jackson provides quality secondary antibodies that are highly affordable with more conjugation options than any other vendor I'm familiar with. We use both their biotin- and fluor-conjugated antibodies routinely for IHC & IF and have never experienced any issues. Not only do the antibodies perform well, but the technical staff is exceptional; I have learned much from them over the years. They're definitely my go-to for secondary antibodies.
Review Date: 25 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Cancer research
I have a limited number of antibodies from Jackson, but the quality of these antibodies is good.
Review Date: 24 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
FLOW CYTOMETRY
GREAT PRODUCT! It has shown reproducible results for application in flow cytometry staining.
Review Date: 23 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Immunohistochemistry
I achieve perfect results. Antibodies are easy to use. Dilution for majority of the antibodies is the same that I appreciate. Rich offer of different antibodies, inc. various subclasses. Unfortunately, I miss e.g. secondary anti-rat IgG2a antibody.
Review Date: 23 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Testing of antibodies on sections of animal tissues
We use already for at least twenty five years the secondary antibodies of Jackson ImmunoResearch Laboratories. We use the IgG purified antibodies but also the other types of antibodies supplied by them. The quality of the products is excellent and they are easy to keep in the -20°C freezer. Because they can be kept liquid at -20°C by adding glycerol there is no danger of degrading. We are very satisfied and they are good value for money.
Review Date: 23 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Immunostaining of brain sections
We have been using Jackson secondary antibodies for years. They are also good.
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Immunohistochemistry and immunofluorescence
Great quality product with minimum non-specific background. Worth the money spend
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Suitable for the majority of immunodetection procedures.
- Detect mouse, rabbit, rat, human, goat, hamster, bovine, cat, chicken, dog, guinea pig, horse, sheep and swine antibodies.
- Comprehensive specificity options including, heavy and light chain, Fc specific, F(ab')2 specific
- Anti-Mouse IgG subclass specific antibodies
- Light-chain specific antibodies for western blotting after immunoprecipitation
- Anti-Digoxin, anti-biotin, anti-FITC and anti-HRP for signal enhancement
- Fluorescent conjugates covering commonly used excitation sources and filter sets, including Alexa Fluors®, DyLight™, Cy™ Dyes, FITC and more.
- Biotinylated and enzyme conjugates (HRP, Alkaline Phosphatase)
- R-PE, APC and PerCP conjugates for flow cytometry
- Alexa Fluor® 680 and 790 conjugates for high sensitivity western blots
- Colloidal gold complexes for electron and light microscopy