Cellecta’s 80K Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we hav…
The DriverMap™ Human Genome-Wide Targeted Expression Profiling Assay enables researchers to simultaneously measure the expression level of almost 19,000 human protein-coding genes in a single assay. By combining highly multiplexed RT-PCR amplification with the depth and precision of next-generation sequencing (NGS) quantitation, the Driver-Map assay provides convenient, comprehensive, highly sensitive, and quantitative meas…
Cellecta’s LINE-1 Assay Kit provides a convenient, efficient, and targeted Next-Generation Sequencing (NGS) based approach to identify polymorphic insertion sites of LINE-1 in the human genome. The LINE-1 Assay Kit includes all the components needed to detect and identify novel LINE-1-induced genomic variations in genomic DNA isolated from tumors, blood, cells or other samples. Only a few hundred nanograms of DNA are requir…
Want to know if Cas9 is active in your cells? Cellecta’s CRISPRtest™ method allows you to assess the level of Cas9 activity in your cell line using two pre-mixed lentiviral-packaged vectors, the first containing a green fluorescent protein (GFP) marker and gRNA sequence targeting an essential gene (PCNA), and the second containing a red fluorescent protein (RFP) marker and a non-targeting gRNA. Transduction of CRI…
Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors. The Packaging Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the necessary structural, regulatory, and replication proteins required to…
LentiFuge™ viral concentration provides a simple, rapid, and effective method to increase the amount of lentiviral particles collected from cell culture supernatants using standard high-speed centrifugation. It works by aggregating viral particles so that upon high speed centrifugation, so the virus can be precipitated and isolated without use of an ultracentrifuge. LentiFuge is a sterile, cell culture grade reagent that is…
Easily label cells with an RNA-expressed barcode readily detected by RNA-Seq analysis
The DriverMap Predesigned Targeted Expression Profiling Panels are pooled sets of experimentally validated primers to specifically amplify over 1200 expertly curated genes whose expression provides insight on a variety of key pathways.
