Merck Products & Reviews

Pooled CRISPR screening with 10x Genomics compatibility

Easier nucleic acid preparation with fewer impurities.

Easier nucleic acid preparation with fewer impurities.

from Saccharomyces cerevisiae, ≥99%.

Nuclease-free water, for molecular biology

Powder, γ-irradiated, BioXtra, suitable for cell culture.

Lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology.

Supel™ Swift HLB is a polymeric stationary phase for solid phase extraction (SPE) prior to instrumental analysis. It has both hydrophilic and lipophilic functional groups for the extraction of a broad range of compounds from aqueous samples. It retains analytes having diff erent polarities and Log P values due to its hydrophilic and lipophilic balance (HLB) property.

Our Smart SPME fibers are equipped with a unique Smart chip that offers traceability, ease-of-use, and increased productivity. The smart SPME fibers are an extension of our expertise and innovation from traditional SPME coatings to smart SPME format for accurate, reliable, and consistent results.

The over coating of fibers is a new technology in fiber coating to protect the SPME fiber from matrix components. This allows for immersion of the fiber directly into complex samples ensuring a more efficient extraction (e.g. of semivolatile compounds). Polydimethylsiloxane (PDMS) is used to overcoat a PDMS/divinylbenzene (DVB) fiber. The resulting fiber is more physically robust and resistant to chemical fouling and allows f…

The nitinol-core of SPME fibers enhances the fiber performance and the precision of chromatographic results to enhance lot-to-lot and fiber-to-fiber reproducibility, increase fiber durability, and maintain selectivity and inertness compared to fused silica core fibers.

Pooled and Arrayed Genome-Wide LentiORF Libraries for High-Throughput Screens or Targeted Experiments

Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

Trusted TRC (The RNAi Consortium) content with shRNA targting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma

We have applied CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.

CompoZr ® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes to create reporter cell lines for compound screening

Enable assessment of drug transport by comparison between the wild-type (WT) and knockout cell lines

A pre-engineered cell line with integrated genetic elements for simple payload exchange of your specific promoter and gene of interest. The landing pad construct is targeted to the AAVS1 safe harbor locus with a fluorescent marker for downstream screening.

The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018)

The Human Whole Genome Toronto KnockOut Library v3 (TKOv3) is optimized for editing efficiency using empirical data described in the findings by the Moffat lab.

The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions.

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD)

The Human CRISPR inhibition (CRISPRi) library (Dolcetto) is a single compact library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening using a KRAB-dCas9 effector.