The Thermo Scientific 96-Well Semi-Skirted Plates, Fast Block hold a maximum of 0.1 mL of well volume when used in conjunction with adhesive and heat seals. The plates are directly compatible with all Applied Biosystems thermal cycler fast blocks, no adapters necessary. These semi-skirted low profile thin walled plates feature a raised deck to aid in spill prevention and come with a cut corner at the A1 location. The 96-Well S…
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Random Decamers are truly random oligo deoxyribonucleotides that can be used to create a pool of cDNAs for subsequent applications, thus providing maximum flexibility.
Thermo Scientific Random Hexamers are truly random oligo deoxyribonucleotides. Hexamers are ideal primers for mRNA with or without a poly-A tail. Hexamers bind more often than decamers, giving higher yields and increased sensitivity when amplifying short fragments.
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Magnesium ions (Mg2+) are an important component of PCR reactions where they interact with the DNA template, dNTPs, and Taq DNA Polymerase.
Adhesive film and foils seals for PCR and qPCR plates. Gas permeable seals and crystallography seals. Adhesive PCR Film Seal:Thermo Scientific Adhesive PCR Plate Seals are a reliable method of sealing plates for PCR applications. The high integrity sealing materials give the best protection against evaporation for high efficiency PCR. Highlights • Clear for sample inspection • Strong adhesive prevents evaporation during therm…
Power SYBR® Green RNA-to-CT 2-Step Kit is a flexible kit that enables a two-step protocol for performing reverse transcription and cDNA quantitation by real-time PCR. The kit includes the High Capacity RNA-to-cDNA and the PowerSYBR® Green PCR Master Mix. The High Capacity RNA-to-cDNA Kit is used to first reverse transcribe RNA into cDNA and the resulting cDNA is then quantitated using the PowerSYBR® Green PCR Master Mix for…
Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.
Ambion Pre-miR™ miRNA Precursor Molecules are small, chemically-modified double-stranded RNA molecules designed to mimic endogenous mature miRNA molecules. These ready-to-use miRNA mimics, which are similar to but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters identical to those used for siRNAs and enable detailed study of miRNA biological effects via gain-of-function e…
Silencer Pre-Designed siRNAs are Ambion-designed siRNAs available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information. Silencer Validated siRNAs are Ambion-designed siRNAs that have been…
Ambion Silencer Select Pre-Designed and Validated siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Ambion Silencer® In Vivo Ready siRNAs undergo the additional purification and testing necessary for introduction of siRNA into animals. After HPLC purification and annealing, each siRNA is dialyzed to remove salt, sterile filtered, and endotoxin tested. All Silencer Pre-Designed and Validated siRNAs are available in this format, as are Custom Synthesized siRNAs.
Abmion Validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs are available for human, mouse and rat with the same chemical modifications for enhanced efficacy as in other Ambion Silencer Select siRNAs.
For the first time, you can obtain the benefits of Ambion Silencer Select siRNAs in a convenient 96- or 384-well plate format, plated to your specifications. These siRNA collections enable medium- and high-throughput analysis of phenotypes resulting from inhibition of sets of targets from specific functional classes or your favorite list of targets.
Silencer siRNA Libraries are sets of Ambion-designed siRNAs targeting all of the genes in a genome or a particular gene class, making them ideal for pathway analysis and target identification/validation. Most libraries include three individual siRNAs per gene for enhanced data reliability. Full sequence information is provided with each library.
Applied Biosystems currently offers five TaqMan Copy Number Assays to detect copy number variation. Copy number is an important polymorphism in the human genome associated with genetic diseases such as cancer, immune diseases, and neurological disorders. TaqMan Copy Number Assays were designed to detect CYP2D6, CYP2A6, CYP2E1, GSTT1, and GSTM1.
The TaqMan® Custom Array is a 384-well micro fluidic card that enables a researcher to perform 384 simultaneous real-time PCR reactions without the need to use liquid-handling robots or multichannel pipettors to load. This low- to medium- throughput array allows for 1-8 samples to be run in parallel against 12-384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the array. The TaqMan Custom…
The TaqMan Custom Plating service offers the convenience of pre-plated TaqMan® Gene Expression Assays, Custom Assays, and Custom Probe/Primer Sets in 96- or 384-well plates. This cost-effective custom plating service gives you the flexibility and reliability you need for your screening applications. Whether you are looking for biomarkers, testing for toxicity, searching for microbes, or performing other high-throughput scree…
Applied Biosystems TaqMan Drug Metabolism Genotyping Assays are available for basic research and clinical research. Genotype over 2,600 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes with TaqMan® Assay technology.
TaqMan® Express Plates are a cost-effective and convenient 96-well format complimenting Applied Biosystem's line of individual TaqMan® Gene Expression Assays, and pre-spotted 384-well TaqMan® Arrays. Customize any of the six available plate formats with dried-down TaqMan® Assays by positioning each in specific well locations. This flexibility enables an efficient means to screen biological samples for the presence and amount…
TaqMan® Gene Sets are pre-defined lists of genes grouped by pathway or disease target class which can be ordered separately, as a custom plate, or configured directly onto TaqMan® Arrays to enable you to perform hundreds of real-time PCR reactions simultaneously on the Applied Biosystems 7900HT Fast Real-Time PCR System. Simple to implement, TaqMan Gene Sets are customizable, and when placed on a TaqMan Array, require mini…
TaqMan® Gene Signature Arrays enable analysis of hundreds of TaqMan® Gene Expression Assays on a 384-well micro fluidic card with minimal effort. TaqMan Gene Signature Arrays are readily available and delivered 2–4 days after ordering. They are packaged conveniently with two or four arrays per pack, making them cost effective, and just the right size for any experiment. Choose from pathway-specific pre-defined arrays such…
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results. Get gold standard TaqMan Assay performance for miRNA quantitation and profiling…
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
TaqMan® PreAmp Pools are pre-pooled sets of Applied Biosystems TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are availab…
The TaqMan Sample-to-SNP Kit provides a streamlined protocol for performing TaqMan® chemistry-based genotyping analysis from any sample with a single kit. The kit is comprised of two parts: the DNA Extract All Lysis Reagents and the TaqMan® GTXpress™ Master Mix. The DNA Extract All Lysis Reagents reduce prolonged procedures for the release of real-time PCR-ready DNA to a 5 minute protocol. They can process a wide variety of…
