Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…
Thermo Scientific IPTG (isopropyl-ß-D-thiogalactopyranoside) is a chemical analogue of galactose, which cannot be cleaved by ß-galactosidase. It acts as an inducer of the lac operon.
Full-, semi- or non-skirted 96-well PCR plates with different profiles. Ultra-rigid plates for robotic applications.
Thermo Scientific Anchored Oligo-dT primers are specifically designed for synthesising long cDNAs from mRNAs carrying poly-A tails. Since this primer only anneals to the poly-A tail junction of the mRNA template, it eliminates transcription through the poly-A tail, providing more effective cDNA synthesis.
dNTP mixes – supplied in a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP. Thermo Scientific produces extremly pure dNTPs, and the company is one of only a few primary manufacturers in the industry. Thermo Scientific dNTPs are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually as nucleotide mixes at convenient concentrations for direct use in PCR, reverse transcription, label…
The LCQ Fleet delivers rich information for routine analysis of complex samples with high-throughput and provides excellent full-scan sensitivity, ruggedness and reliability. From the inventors of benchtop LC/MS ion trap technology, this reliable and easy to use ion trap mass spectrometer offers exceptional analytical value while providing confident sample analysis. The new cost effective LCQ Fleet delivers rich information f…
The Power SYBR® Green RNA-to-CT™ 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that are common in SYBR® Green-based assays. This significantly improves data accuracy and enables the reliable detection of low expressing targets or targets present at low amounts. Minimized hands-on time wit…
The SYBR Green RT-PCR Kit provides all the necessary components to perform both one- and two-step RT-PCR, which makes it ideal for evaluation between the two RT-PCR protocols.
The Thermo Scientific Total RNA Isolation Reagent (TRIR) is designed for the extraction of total RNA from a variety of sources. A biological sample is homogenised with the reagent using a glass-Teflon® homogeniser. After extraction with chloroform, the homogenate separates into two phases. RNA remains in the aqueous phase while DNA and proteins are extracted into the organic phase. The RNA is precipitated from the aqueous phas…
The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…
