Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
This kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for kit is up to 500 mg respectively. This kit can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified p…
This kit provides a rapid and economical method for the purification of plasmid DNA in a convenient mini column format. Each AxyPrep column has a binding capacity of at least 20 mg of plasmid DNA. AxyPrep columns are designed for use in either a spin or vacuum format. Each column can process up to 4 ml of bacterial culture grown in LB or up to 2 ml of culture grown in rich broth. The entire procedure can be completed within…
AxyPrep™ Multisource Genomic DNA Miniprep Kit is designed to purify genomic DNA from animal and plant tissues, cultured cells and yeast. Proteins, pigments, carbohydrates and lipids are efficiently segregated from the genomic DNA by a unique two-phase partition of the AxyPrep™ Multisource Genomic DNA Miniprep Kit. The genomic DNA is bound to an AxyPrep™ Genomic DNA column, where residual impurities and salt are removed. Geno…
R&D Human TNF-alpha/TNFSF1A Quantikine ELISA Kit - Human TNF-alpha Immunoassay for the quantitative determination of human tumor necrosis factor alpha (TNF-alpha) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human TNF-alpha Immunoassay is a 3.5 - 4.5 hour solid phase ELISA. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-alph…
The Guava® Caspase 3/7 and 8 Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspases) reagent that specifically identifies active caspase 3 and 7 molecules or caspase 8 molecules. Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures. Each FLICA is comprised of three subunits: A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD)…
Mouse IL-6 R&D Quantikine ELISA Kit - Mouse IL-6 Immunoassay for the quantitative determination of mouse interleukin 6 (IL-6) concentrations in cell culture supernates, mouse serum, and plasma. The R&D Quantikine Mouse IL-6 ELSIA is a 4.5 hour solid-phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique.R&D Systems Quantikine ELISA Kits are complete and ready-to-use. Features of…
R&D Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit - R&D Mouse IL-1/IL-1F2 Immunoassay for the quantitative determination of mouse interleukin 1 beta (IL-1ß) concentrations in cell culture supernates and mouse serum. The R&D Quantikine Mouse IL-1 Immunoassay is a 4.5 hour solid phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique. Features of the R&D Quantikine ELISA Kit inclu…
C Technologies, Inc.'s new variable pathlength extension enables UV/Vis measurements of varying solution concentrations without the need for dilutions. The closed loop system dynamically changes the measurement pathlength based upon user specified thresholds and criteria. Pathlength Modes: - Specific Pathlength Single pathlength operation - Discrete Pathlengths Multiple user specified pathlengths - Pathlength Scanning User Def…
More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular. Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors…
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemilu…
The NL100 Nitrogen Laser is ideal for fluorescence measurements, MALDI-TOF mass spectrometers, and other pulsed UV radiation experiments. It provides 3.5 ns pulses at 337 nm (UV), with repetition rates up to 20 Hz. The pulse energy is 170 µJ, which results in a peak power of 45 kW and an average power of 3 mW. The NL100 can be triggered internally or externally at rates up to 20 Hz. It can also provide a sync output pulse (opt…
The StellarNet EPP2000 Spectrometers are miniature fiber optic instruments for UV, VIS, and NIR measurements in 190-1700nm ranges. Each unit contains a high speed parallel digitizer interface with a 4K word spectral scan memory to provide instantaneous spectral data from the highly sensitive CCD or Photo Diode Array detectors. Various models provide a choice of grating range and slit resolutions. A single strand fiber or probe…
The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time! Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d. system applies a vacuum to actively drive reagents through the membrane. This novel method allows you to optimize your blotting conditions in record time for maximum results. The SNAP i.d. system minimizes over…
The MICROLAB® NIMBUS is Hamilton Company's newest automated multi-channel pipetting platform, offering a high density deck layout in a body small enough to fit inside a laminar flow hood. The system utilizes the same highly accurate 96-channel pipetting head as Hamilton's STAR instruments, yet is designed for smaller throughput applications. The NIMBUS is ideal for: • Fast plate replications • Row-wise and column-wide serial d…
Each VECTASTAIN® ABC kit utilizes the same preformed Avidin: Biotinylated enzyme Complex (ABC) principle and all offer low backgrounds and an amplification of signal compared to conven-tional covalent conjugates. The high sensitivity reported for this technique likely resides in either the form or the number of active enzyme molecules associated with the complex and the rapid, irreversible interaction of the complex with bio…
The mirVana miRNA Isolation Kit uses a rapid procedure to isolate small RNAs, such as microRNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells. The fast and efficient glass fiber filter (GFF) based method isolates total RNA ranging in size from kilobases down to 10mers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, w…
Fill-It - is an automated benchtop system for filling screw-cap cryovials. Fill-It is designed to help create cell banks of higher quality, consistency and viability. It combines proven decap / recap modules with aseptic liquid handling. Fill-It removes all screw caps simultaneously and then dispenses into multiple cryovials in parallel. Compatible with Corning, Greiner, Micronic, Matrix, Nalgene, Nunc and Sarstedt screw-cap c…
The Thermo Scientific TSQ Quantum Ultra mass spectrometer provides ultimate productivity for bioanalytical analysis. Faster method development at the highest possible specificity is routinely achievable with the TSQ Quantum Ultra™ mass spectrometer. Featuring the patented Thermo Scientific HyperQuad quadrupole mass analyzer system, the TSQ Quantum Ultra performs highly selective reaction monitoring (H-SRM), which provides gr…
Your benchmark for fluorescent protein flow cytometry
High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. The MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed…
The new MultiGene™ OptiMax replaces our successful and reliable MultiGene™ Gradient thermal cycler and brings new speed and advanced features to our Labnet Thermal Cycler line providing premium performance at an affordable price. • Unique Flexible Programming with FlexTemp™ technology • Fast ramp rates 5oC heating / 3.5oC cooling • Simple user interface • No condensation after overnight cooling at 4°C • 3 Year Warranty The Mul…
The MagNA Pure 96 System from Roche Applied Science has been designed and engineered to meet the needs for high throughput laboratories worldwide. The integrated system enables batch processing of up to 96 samples in parallel ensuring every sample is treated exactly the same while providing extremely quick turnaround time. These characteristics result in a low sample processing time; for typical standard applications, 96 sampl…
The TProfessional Basic Thermocycler features a gold anodized 96 well block or an anodized 96 well XL block (NEW!!!) for extra large volumes up to 100µl. The gold anodized 96 well block is designed for standard applications whereas the XL block is especially developed for special protocols where large volumes are required like emulsion PCR for new generation sequencing. Both block types offer an excellent temperature uniformit…
The Thermo Scientific™ Evolution 60S UV-Visible spectrophotometer offers exceptional performance and flexibility for routine to research applications. With a 1.0 spectral bandwidth, the system provides increased resolution for your most demanding samples.Evolution™ 60S UV-Visible Spectrophotometer Features: Acquire accurate spectra with scan speeds up to 4200nm/min. Depend on dual-beam optics for superior photometric ac…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
