Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
The Power SYBR® Green RNA-to-CT™ 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that are common in SYBR® Green-based assays. This significantly improves data accuracy and enables the reliable detection of low expressing targets or targets present at low amounts. Minimized hands-on time wit…
The SYBR Green RT-PCR Kit provides all the necessary components to perform both one- and two-step RT-PCR, which makes it ideal for evaluation between the two RT-PCR protocols.
The Thermo Scientific Total RNA Isolation Reagent (TRIR) is designed for the extraction of total RNA from a variety of sources. A biological sample is homogenised with the reagent using a glass-Teflon® homogeniser. After extraction with chloroform, the homogenate separates into two phases. RNA remains in the aqueous phase while DNA and proteins are extracted into the organic phase. The RNA is precipitated from the aqueous phas…
The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…
The Veriti™ 384-Well Thermal Cycler (0.02 mL) delivers the proven reliability of Applied Biosystems 9700 GeneAmp® instruments with the throughput you need for large experiments. • Network up to 12 instruments together using standard Ethernet cables and a router (not provided) • From a single, networked Veriti™ instrument, start a run on up to 12 Veritis • Conveniently place instruments side-by-side due to front to back venti…
The Veriti™ 60-Well Thermal Cycler delivers the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® instruments with the 0.5 mL format you need to accomodate larger reaction volumes. . Additionally, the powerful user interface through the color touch screen simplifies the setup and use of the Veriti 60-Well Thermal Cycler. Optional setups for fast or standard PCR methods provide you the flexibility to shorten your…
Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex Primer Pools deliver comprehensive coverage of Sanger miRBase v10, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution. Optional Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs en…
The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging in size from 300 bp to 10,000 bp. This marker is ideal for the size determination of digested DNA. Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crips band patterns. The two m…
The 100 bp DNA Ladder contains 12 discrete DNA fragments ranging in size from 100 bp to 3,000 bp. This marker is ideal for the size determination of PCR products. Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crisp band patterns. The two…
For purification of up to 20 µg molecular biology grade plasmid DNA
AxyPrep Bacterial Genomic DNA Miniprep Kit - Suitable for the rapid isolation of up to 20 mg of genomic DNA from 1.0×109 bacterial cells. Following lysis, separation of the genomic DNA from proteins, polysaccharides and lipids with the AxyPrep Plasmid MiniPrep Kit is achieved by a unique phase-partition step. The genomic DNA in the lower phase is selectively bound to a special Axygen AxyPrep column for further purification a…
Format of the AxyPrep™ Multisource Total RNA Maxiprep Kit: • Maxi column AxyPrep™ Multisource Total RNA Maxiprep Kit Starting Materials: • Animal tissues • Plant tissue • Cultured cells • Bacteria • Yeast • Filamentous fungi Features of the AxyPrep™ Multisource Total RNA Maxiprep Kit: • Rapid spin and vacuum protocols • No alcohol precipitation • No column clogging • Dramatic reduction in DNA carryover • Higher yields, higher…
R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays…
Human VEGF Quantikine ELISA Kit - Human VEGF Immunoassay for the quantitative determination of human vascular endothelial growth factor (VEGF) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human VEGF ELISA is a 4.5 hour solid phase ELISA. This R&D ELISA employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VEGF has been pre-coated onto a microplat…
Millipore’s The FlowCellect™Rodent Neural Stem Cell Characterization Kit for Neuronal Differentiation is designed as an easier and quicker way to track neuronal differentiation. The kit will also help to determine the percentage of undifferentiated rat neural stem cells in culture by determining the percentage of cells that express both Sox-2 and Nestin as well as track Beta-III-Tubulin upregulation upon Neuronal differentiati…
Protein fractionation, in-solution recovery Prefractionation of proteins and peptides prior to LC/MS analysis can dramatically increase the number of proteins ultimately identified. The Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pI-based fractionation. The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels.…
UltraTrol™ is offered in several formulations that allow the user to select the appropriate UltraTrol™ dynamic pre-coating to optimize their separation outcomes, within the pH and buffer requirements stipulated by the run buffer. In addition to being relatively independent of pH and buffer selection, a critical value delivered by UltraTrol™ is the outstanding reproducibility of separation control that it provides for the selec…
R&D GM-CSF -Recombinant Mouse Protein GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types in response to cytokine or inflammatory stimuli. Specifications and Use for R&D GM-CSF Source of R&D GM-CSF • A DNA…
The IsoPrime is a high performance gas source stable isotope ratio mass spectrometer designed to operate in dual inlet or continuous flow modes of operation. The modules are designed to handle samples such as solids, liquids or gases and prepare them to be introduced into the ion source of the IRMS as purified gases. Resultant prepared gases such as (H2, CO2, CO, N2, SO2 or N2O) are then ionised and analysed for their isotopic…
KOD Hot Start DNA Polymerase is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3´→ 5´ exonuclease activities at ambient temperatures. KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can oc…
