Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
The Concanavalin A (Con A) Solution (500X) is a ready-to-use solution of Con A in aqueous buffer. Con A, isolated from the Jack bean, Canavlia ensiformis, is a lectin that binds alpha-D-glucose and alpha-D-mannose moieties found in various glycoproteins, glycolipids, and sugars and is a potent leukocyte mitogen. This reagent is intended for use in in vitro activation of human and mouse leukocytes.
The Protein Transport Inhibitor Cocktail (500X), a cocktail of Brefeldin A and Monensin, is used for assays in which prevention of protein transport to the extracellular space is desired and has been specifically formulated for use as a negative control for the Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (cat. 00-4975). Addition of this cocktail to cells in culture inhibits the intracellular protein se…
The Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent solutions are used for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. It should be used in combination with the Permeabilization Buffer (10X) (cat. # 00-8333).
The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
The Super Bright Staining Buffer is a supplement to Flow Cytometry Staining Buffer for immunofluorescent staining protocols of cells in suspension. Super Bright Staining Buffer prevents non-specific polymer interactions when more than one SuperBright conjugated antibody is used in the same experiment, which otherwise could lead to data appearing under-compensated. The buffer is provided in a convenient 5 μL/test format and is…
The 2-component FIX & PERM Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.FeaturesFlexible—compatible with multiple sample types and cellular antigensRobust—scatter pattern of cells unaffectedEfficient—simultaneous detection of intracellular and surface markersThe FIX & PERM f…
Cal-Lyse solution is a lysing solution formulated for the lysis of erythrocytes in samples of anti-coagulated human peripheral blood. Cal-Lyse lysing solution can be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for flow cytometric analysis.
High-Yield Lyse solution, premixed, fixative-free erythrocyte lysing solution, can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. The solution immediately lyses erythrocytes following staining of the blood samples with fluorochrome-conjugated monoclonal antibodies
CellEvent Caspase-3/7 Green Ready Flow Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteExceptionally bright green fluorescen…
The Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…
