Life sciences encompass a diverse range of scientific disciplines focused on the study of living organisms and their interactions with the environment. From basic life sciences (such as cell biology, genetics, immunology, and microbiology) to applied life sciences (such as disease research, multi-omics, and biotechnology), this broad field explores the fundamental processes of life and drives advancements in disease research, patient care, and our overall understanding of living organisms. Discover the endless possibilities of life sciences and their impact on shaping a healthier and more sustainable world.
The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…
Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cyto…
Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents and agents to stabilize their activity on cells. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to…
ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retain…
EndoGRO Human Umbilical Vein Endothelial Cells (HUVEC) are a commonly studied human cell type extracted from human neonatal umbilical cords. They are relatively easy to culture, and provide a valuable cell model for many vascular biology research applications, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction, and vasodilation. EndoGRO™ HUVEC are provided at passage 1, and have been qualit…
ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.
ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will…
ReNcell Neural Stem Cell Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX and VM cultured in serum-free conditions with ReNcell NSC Maintenance Medium. The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.
ENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (90% bIII-tubulin positive) are obtained. Very low levels of…
HEScGRO hES cell medium is the first, animal-component free medium that is specially formulated to meet the unique requirements of human embryonic stem cell culture. No other hES cell media is available as a serum-free, ready-to-use, and complete formulation. HEScGRO has been extensively tested and proven to maintain the pluripotential nature of several hES cell lines including: MEL-1, MEL-2 and H1. This medium is fully-define…
A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes.ACCUMAX™ is tested for Sterility, enzymatic activity and cell detachment from tissue culture plastic. ACCUMAX™ has been used successfully on numerous cell types including: CHO, BHK, 293, COS, Sf9, and catfish primary hepatocyte cells.
This cell line arose from a primary culture of dispersed human fetal pancreatic cells. A single pancreas gland at 18 weeks gestation was dispersed by micro-dissection and collagenase digestion (2 mg/ml for 50 minutes at 37°C). Following washout of enzyme, dispersed cells were incubated with magnetic beads coupled to monoclonal antibodies specific to CD90 though a DNA linkage. Magnetic isolation and the detachment of the magnet…
Embryonic Stem Cell Lines derived from murine 129/SVEV selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 11.
Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 11.
This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data)and is ES cell qualified. Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal Bovine Serum. The reagents used in these products are certified to be virus and mycoplasma free. The sera ut…
The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS). Such sera must be carefully controlled and tested. Specialty Media utilizes sera that are approved by the United States Department of Agriculture (USDA) to ensure that sera are free of diseases such as Bovine Spongiform Encephalopathy (BSE). A Certificate…
The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS). Such sera must be carefully controlled and tested. EMD Millipore utilizes sera that are approved by the United States Department of Agriculture (USDA) to ensure that sera are free of diseases such as Bovine Spongiform Encephalopathy (BSE). A Certificate of…
We now offer our most popular embryo culture media in a dry powder format. Each has been formulated for optimal, consistent performance. This new dry powdered format offers greatly increased shelf-life, easy and quick preparation, and the freshest nutrients to support the development of your embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with…
Dry Powdered form offers easy and quick preparation, and the freshest nutrients to support the development of embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with the correct pH and osmolarity.
dry powder format. Each has been formulated for optimal, consistent performance. This new dry powdered format offers greatly increased shelf-life, easy and quick preparation, and the freshest nutrients to support the development of your embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with the correct pH and osmolarity.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
PMEF-CFL are Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:
PMEF-HL are Hygromycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded before freezing for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
