Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
R&D M-CSF - Recombinant Mouse Protein M-CSF, also known as CSF-1, is the primary regulator of macrophage survival, proliferation and differentiation and is essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis.…
R&D PDGF BB, CF - Recombinant PDGF-BB Human Protein Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the α-granules of platelets activated during the coagulation of blood to form serum. Specifications and Use of R&D PDGFSource of of R&D PDGF • A DNA sequence encoding the 109 amino acid residue mature human PDGF-B chain p…
R&D Systems - Recombinant Human Bone morphogenetic protein 2 (BMP-2) Bone morphogenetic protein 2 (BMP-2)is a member of the BMP subgroup of the TGF-β superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration.Specifications and Use of R&D BMP-2R&D BMP-2 Source • The DNA sequence encoding human BMP-2 (Wozney, J. et al., 1988, Science 242…
R&D Systems - Recombinant Human HGF (NS0-expressed)HGF, also known as Hepatopoietin A, was initially identified as a potent mitogen for hepatocytes in primary culture. Subsequently, HGF was also shown to be mitogenic for a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. Specifications and Use of R&D HGFSource of R&D human HGF • A DNA sequence encoding the 728 amino acid residu…
R&D Systems Recombinant Human IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1α (IL-1F1) and IL-1β (IL-1F2), which are the products of distinct genes. IL-1α and IL-1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. S…
R&D Systems Recombinant Human IL-2 Interleukin-2 (IL-2) is a O-glycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. Specifications and Use of R&D IL-2 Source • A DNA sequence encoding the mature human IL-2 protein sequence (Taniguchi et al., 1983, Nature 302: 305 - 310; Ma…
R&D Systems Recombinant Human FGF basic (157 aa)FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 - 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. This factor contains four cysteine residues, but reduced FGF basic retains fu…
TGF- β1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-β superfamily that share a characteristic cystine knot structure.1 - 7 TGF-β1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Specifications and Use for R&D TGF-beta1Source •…
R&D Systems Recombinant Human HGFHGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Specifications and Use of R&D HGF Source of R&D HGF• A DNA sequence encoding the 728 am…
Cell Culture Inserts • For multidishes 6, 12, 24 • Treated and tested for cell attachment • Easy cultivation of most cell types without matrix coating • Used in a varity of applications, including transport studies, toxicity tests, chemotaxis studies and electron microscopy • Non-toxic • Packed in sterile Nunclon™Δ treated multidishes • Packed in resealable bags • Non-pyrogenic • Tabs for better grip/handling
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Exiqon miRCURY LNA™ microRNA Knockdown Probes High-affinity miRCURY LNA microRNA Knockdown Probesfor specific in vitro knockdown of microRNAs. Featuring minimal cytotoxicity and high biological stability. Key features of miRCURY LNA™ microRNA Knockdown Probes include:• Effective and sequence-specific antisense inhibition of microRNAs • Probes available for all known microRNAs • Superior biological stability • Minimal cytotoxi…
New Release 2009! 3D Prime Culture Medium. Improving still further our originial medium for 3D culture of epidermal keratinocytes, CELLnTEC has now released a new formulation, known as 3D Prime.Developed using our established signalling-based approach, this completely defined medium enables more rapid establishment of a fully stratified epidermis. Using this medium, models can now be ready for experimentation in just 14-18 day…
Fits plastic or glass media bottles with 45mm neck sizes. Cellulose acetate (CA) membranes provide fast flow rates and low protein binding and are good for filtering cell culture media. Angled hose connector simplifies vacuum line attachment. Individually packaged, sterile and certified nonpyrogenic. Each system has the membrane material and pore size printed on the unit and is color-coded by membrane type for easy produ…
CnT-27 is a progenitor cell targeted (PCT) liquid culture medium kit including both basal medium and separate supplements, developed for improved isolation and growth of mammary epithelium. CnT-27 is fully defined, serum and BPE-free formulation. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
Gibco Geltrex Matrix is a soluble form of reduced growth factor (RGF) basement membrane extract (BME) purified from murine Engelbreth-Holm-Swarm tumor
Optimized to achieve over 50% transfection efficiency without sacrificing cellular health, each kit includes an optimized protocol, Prostate Reagent and Prostate Boost Reagent in sufficient quantities to conduct up to 500 transfections in 35mm wells.Features include:• Reduces levels of cell damage when compared to liposmal-based transfection reagents • Transfection can be conducted in serum-containing media to save time and el…
R&D Fluorokine Receptor Detection Kits - Biotinylated ligands for estimating receptor densities R&D Systems now offers a Human Activin A Biotinylated Fluorokine Kit (Catalog # NFACTA). This flow cytometry kit is designed for the detection of Activin A receptor-expressing cells by flow cytometry. Activin A is a disulfide-linked homodimer belonging to the TGF-beta superfamily that signals through Type I/Type II receptor comple…
R&D Mouse Obesity Antibody Array - Proteome Profiler Array - For the parallel determination of the relative levels of selected mouse Adipokines. R&D Mouse Obesity Array (Catalog # ARY013) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary. R&D Mouse Obesity Antibody Array for the meas…
ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency. Suitable fo…
The Neurite Outgrowth HCS Reagent Kit product provides a more effective solution for quantifying neurite outgrowth by combining high-content screening (HCS)-quality fluorescence reagents with a validated, automation-compatible protocol. Neurite Outgrowth Reagent Kit Features: Neuronal-specific fluorescent reagent identifies neuronal cell bodies and neurites in a heterogeneous cell population without isolation steps Val…
LifeXtend™ conjugate stabilizer/diluent is a proprietary multi-component reagent system that stabilizes antibody-HRP conjugates both at 4°C and at ambient temperature. By using LifeXtend™ HRP stabilizer/diluent in antibody conjugation the HRP conjugate can be stabilized during use and stored as pre-diluted, ready-to-use reagents thus eliminating waste and improving consistency from experiment to experiment. LifeXtend™ shows…
SIRT1 belongs to the class III family of HDACs, which are NAD+ dependent enzymes. SIRT1 modulators have been shown to play a beneficial role in treating diseases such as cancer, diabetes, metabolic disease and inflammation, making them attractive targets for the development of new drugs. The new HTRF® SIRT1 assay, developed by Cisbio Bioassays using its patented HTRF technology, is a homogeneous method for directly measuring S…
Specially designed for use with the Cecil Adept HPLC, Q-Adept HPLC, Merit HPLC and IonQuest ion chromatography.
The Phospho-mTOR Activation Kit measures phosphorylation of mTOR, a kinase involved in the initiation of ribosome biogenesis and translation.
