UHPLC and HPLC Products & Reviews

25

High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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HyperGEL™ SEC Columns

Thermo Fisher Scientific

HyperGEL columns are packed with porous polymeric beads with a precise pore size distribution so that solutes are accurately separated according to molecular size.High efficiency HyperGEL columns provide a minimum of 50,000 plates/metre for 5µm particle size. The columns are available for aqueous (HyperGEL AP) or organic (HyperGEL OP) separations.HyperGEL OP columns are offered in 7 pore sizes and 2 particle sizes to test mole…

4.8/5.0
|3 Reviews

Preparative Columns from Thermo Electron

Thermo Fisher Scientific

Get maximum loading and efficiency from your preparative columns. Achieve high loading with Thermo Electron phases and column sizes up to 50mm ID and 250mm length. Our column packing and silica expertise ensures our preparative columns achieve optimum efficiency and lifetimes.Our BETASIL™ and Hypersil™ 100 phases are ideal for preparative separations. We recommend the separation is proven using an analytical scale column packe…

5.0/5.0
|1 Review

AQUASIL C18 Columns

Thermo Fisher Scientific

o   Excellent retention and alternative selectivity for polar compoundso   Completely stable under 100% aqueous conditionso   Ideal for LC/MS applications Product detail: AQUASIL C18 columns provide more retention for difficult-to-retain polar compounds due to a unique hydrophillic end-capping.  The combination of a moderate coverage C18 group and an additional polar group gives new selectivity compared to a traditional C18.…

5.0/5.0
|1 Review

HyPURITY ADVANCE Columns

Thermo Fisher Scientific

HyPURITY ADVANCE Columns  - offer exceptional chromatographic performance and is ideal for high throughoput laboratories with complex and variable sample types. Product detail: HyPURITY ADVANCE contains a polar amide group embedded within a C8 chain, and is fully endcapped. The columns are based on our metal free HyPURITY silica and, compared to other phases, produces a competely new selectivity. A polar functiona…

4.7/5.0
|1 Review

Hypersil BDS Columns

Thermo Fisher Scientific

Hypersil BDS C18 Columnso   Sharp peaks and improved symmetry for basic compoundso   Value and performance from a globally referenced columno   Long column lifetimes with excellent column-to-column and batch-to-batch reproduciblity Product detail:Hypersil BDS C18 receives a special base deactivation treatment during silica material manufacture to reduce silanophilic activity. Hypersil BDS C18 is endcapped and suitable for chro…

4.7/5.0
|5 Reviews

HyPURITY C18 Columns

Thermo Fisher Scientific

o  Based on ultra pure 190 Å pore size silica for analysis of small molecules to proteinso  Extensive quality control tests to ensure the ultimate in reproducibility and performanceo  Unbeatable peak shapes for all sample typeso  Select from five phases for a wide range of selectivity options Product detail: HyPURITY C18 columns are based on the latest in silica technology - HyPURITY silica, which has less than 10ppm total m…

5.0/5.0
|1 Review

Hypersil™ Amino Acid Columns

Thermo Fisher Scientific

To obtain precise information about the structure of synthetic peptides and proteins, it is imperative to achieve good separation (see illustration).Hypersil Amino acid columns have been developed for this purpose with excellent reproducibility and the highest reproducibility using high grade 5µm particle size silica with a high surface area. Achieve resolution of complex sequences of amino acids with sharp peak shapes.Hypersi…

5.0/5.0
|1 Review

Capillary Columns

Thermo Fisher Scientific

High efficiency  capillaries for high performance, sensitive separations 500, 320, 180, 100 & 75µm ID capillaries  Availabe in a wide range of Thermo phases Rugged sleeved design for robustness 180µm ID flexible KAPPA for FinniganTM ProteomeX™ Workstation Product detail: Capillary and nanobore columns are ideal for applications which require high sensitivity. However, in order to take full benefit of the improvement i…

5.0/5.0
|1 Review

SunFire™ Analytical Columns

Waters

SunFire™ columns feature excellent low pH stability and improved column lifetime compared to many silica-based HPLC column brands. SunFire columns are packed with state-of-the-art silica and new proprietary C18 and C8 bonding and endcapping for high efficiency, resulting in greater sensitivity.  SunFire columns are manufactured to achieve the highest level of chromatographic consistency column-to-column and batch-to-batch. MS-…

4.5/5.0
|3 Reviews

ACQUITY UPLC® BEH C18 and C8 Columns

Waters

ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these…

4.8/5.0
|15 Reviews

ACQUITY UPLC® BEH Shield RP18 Columns

Waters

ACQUITY UPLC® BEH Shield RP18 columns combine Waters patented Shield Technology* with BEH Technology™ by incorporating an embedded carbamate group into the bonded phase ligand.Waters shield ligands consistently demonstrate increased retention of phenolic compounds versus straight chain alkyl columns. The alternate selectivity and excellent peak shape from the embedded polar group ligand, when combined with the wide pH range an…

4.6/5.0
|3 Reviews

Express LC-100

Eksigent Technologies

Run assays up to 5 times faster than a conventional HPLC. Increase mass spec sample throughput by a factor of 5. Eliminate dead time in your analysis and cut solvent usage by 99 percent. Achieve all this and more with the ExpressLC-100 system. TheExpressLC-100 system is a single-channel HPLC with binary solvent delivery, high pressure gradient mixing, variable volume injection, UV absorbance detection, and PC-based instrument…

4.7/5.0
|1 Review



Discovery® BIO Wide Pore C18 HPLC Column

Sigma-Aldrich Supelco

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we h…

5.0/5.0
|1 Review


SecurityGuard™ Universal HPLC Guard Cartridge System

Phenomenex Inc

SecurityGuard offers a convenient and economical way to extend HPLC column lifetimes. Did you know a common cause of high backpressure, split peaks, broad peaks, baseline noise, baseline drift and loss of resolution is contaminants? The fact is all mobile phases contain some chemical contaminants or microparticulates, from the sample, solvent, or wear on the polymeric seals of the pump or injector. These contaminants can…

4.9/5.0
|6 Reviews

GC solution and GCMS solution

LabSolutions is Shimadzu´s software series for chromatography (GC, GCMS, LC, LCMS). The unified graphical interface makes it easy to work with Shimadzu chromatography products. The GCsolution and GCMSsolution is the perfect partner for the Shimadzu GC and GCMS product range. The GCsolution controls the GC-2010 and GC-2014 (from Ver. 2.3) as well as the previous models GC-14 and GC-17. The GCMSsolution Ver. 2 controls the GCMS-…

4.6/5.0
|3 Reviews

Rezex Organic Acid and Carbohydrate Columns

Phenomenex Inc

Rezex™ columns achieve separations based on multiple modes of interaction including ion-exchange, ion-exclusion, size exclusion, reversed phase, and partition. From drug formulation analysis to quality control testing of finished food products, to bioethanol fermentation monitoring Rezex columns consistently provide accurate and reproducible results.Choose Rezex for excellent resolution and reproducibility of sugars, starches…

4.9/5.0
|2 Reviews

Breeze 2 HPLC

Waters

The Breeze™ 2 HPLC System delivers technology and performance in an affordable, compact, and user-friendly system platform. Complete with software, pump, detector and injector, the system comes pre-configured for different levels of HPLC operational needs. From teaching purposes to daily analytical work, the Breeze 2 HPLC System integrates simplicity, sensitivity, accuracy, and reliability. It is ideal for any organization see…

4.5/5.0
|8 Reviews



2489 UV/Visible Detector

Waters

The 2489 UV/Visible Detector is a versatile, dual-wavelength absorbance detector for HPLC. This detector offers the high sensitivity required for routine UV-based applications to low-level impurity identification and quantitative analysis. The 2489 UV/Visible detector’s extended linear range of 2.5 AU makes for easy quantitation of both major and minor components in the same run. You can also simultaneously monitor absorbance…

4.9/5.0
|2 Reviews

2998 Photodiode Array (PDA) Detector

Waters

The 2998 Photodiode Array (PDA) Detector offers advanced optical detection for Waters analytical HPLC, preparative HPLC, or LC/MS system solutions. Providing unprecedented trace impurity detection and quantitation, the 2998 PDA Detector’s integrated software and optics innovations deliver high chromatographic and spectral sensitivity. Enhanced software control provides flexibility for simultaneous 2D and 3D operation with eith…

4.9/5.0
|2 Reviews