Discovery® BIO Wide Pore C18 HPLC Column
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we h…
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Excellent product, I recommend it 100%.
Analyze metabolites from plants extracts
This column shows a high quality and reproducibility of results, the separation of small proteins was very good. This column is ideal for those jobs that require a C18 column. It has excellent reproducibility and the peak shape is exceptional for basic and acidic analytes. It allows very stable and low column bleed LC-MS separations. Separation of peptides and small proteins is performed with great effectiveness, thanks to its lower hydrophobicity than many comparable C18 columns, providing faster analysis.
Review Date: 15 Mar 2021 | Sigma-Aldrich Supelco
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
