Next Generation Sequencing Products & Reviews

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Next-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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PGN004 PureGenome™ Next Generation Sequencing Library Validator Kit

Merck

The quality of sequence obtained from Next Generation sequencing platforms such as Illumina, Inc. requires the accurate determination of input library DNA. Loading too much DNA can lead to overlapping clusters and loss of read counts whereas too little input can lead to poor read lengths and lower sequence output. The PureGenome™ NGS Library Validator Kit measures the absolute amount of adapter-ligated, sequence ready DNA in I…

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Pellet Paint® NF Co-precipitant

Merck

Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has o…

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Strand Specific RNA Library Preparation Kits

Agilent Technologies

The SureSelect Strand-Specific RNA Library Preparation kit prepares Poly(A) enriched libraries for whole-transcriptome sequencing. Sequence a wide range of RNA to detect gene expression changes and alternative splicing. The kit contains mRNA sequencing library preparation and enrichment materials.

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Variant Analysis

Ingenuity Systems Inc.

Identify causal variants from human sequencing data in just hours Rapidly Identify and Prioritize Variants Ingenuity Variant Analysis combines analytical tools and integrated content to help you rapidly identify and prioritize variants by drilling down to a small, targeted subset of compelling variants based both upon published biological evidence and your own knowledge of disease biology. Biological Interpretation of your Var…

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Microlab® NIMBUS NGS workstation

Hamilton Company

Hamilton’s Microlab® NIMBUS NGS workstation provides a completely automated and affordable solution for emPCR emulsion breaking, enrichment and sequencing primer hybridization for the Roche GS Junior System. The fully tested and validated NIMBUS NGS workstation features an integrated REM e system and provides convenient walk-away automation solution to minimize laborious manual process and reduce human errors. The automation p…

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HaloPlex Cancer Research Panel

Agilent Technologies

The HaloPlex Cancer Research Panel enables fast, simple, and efficient analysis of genomic regions of interest for cancer research for a large number of sample types, including FFPE. HaloPlex Cancer is uniquely suited to high performance with cancer research samples, which are commonly preserved as formalin fixed and paraffin embedded (FFPE). This FFPE process often results in highly fragmented DNA, resulting in insufficient…

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GeneSpring NGS Software

Agilent Technologies

Agilent’s GeneSpring NGS software suite includes data analysis workflows for Methyl-Seq, RNA-Seq, DNA-Seq, Chip-Seq and Small RNA-Seq data. GeneSpring NGS provides SureSelect customers with an easy to use QC, visualization and reporting tool for Methyl-Seq, RNA-Seq and DNA-Seq applications. GeneSpring NGS also allows the detection of transcriptomic changes like splice variants and gene fusions, or identify structural variation…

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HaloPlex Exome Kits

Agilent Technologies

HaloPlex technology provides outstanding performance, streamlined workflow, and low sample input requirements for next generation sequencing of human exomes. The HaloPlex Exome has been optimized to provide comprehensive coverage of the coding regions of the human genome with minimal hands on time and requires only 200ng of input DNA. The Haloplex Exome performance and workflow is ideal for clinical research: • Outstanding cov…

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HaloPlex Custom Kits

Agilent Technologies

Agilent Haloplex custom kits offer the flexibility to create custom panels for any genomic regions of interest. A unique and efficient library prep-free workflow enables complete target enrichment in less than 6 hours from just 200 ng of input DNA.

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HaloPlex Cardiomyopathy Research Panel

Agilent Technologies

Agilent HaloPlex panels are predefined designs which focus on targeted gene sets for specific applications. Agilent offers two catalog HaloPlex panels, the Cancer Research Panel and the Cardiomyopathy panel.The HaloPlex Cardiomyopathy (catalog) is a next generation sequencing target enrichment panel designed specifically for inherited forms of cardiomyopathy. Following a careful review of cardiomyopathy publications as well as…

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NEBNext® DNA Library Prep Master Mix Set for 454™

New England Biolabs Inc.

The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation o…

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NEBNext® DNA Library Prep Set for SOLiD™

New England Biolabs Inc.

The NEBNext® DNA Library Prep Set for SOLiD™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library cons…

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent

New England Biolabs Inc.

The NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on an Ion Torrent PGM™. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library…

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

New England Biolabs Inc.

The NEBNext® Fast DNA Library Prep Set for Ion Torrent™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on a Ion Torrent PGM™. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. T…

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NEBNext® Quick DNA Library Prep Master Mix Set for 454™

New England Biolabs Inc.

The NEBNext Quick DNA Library Prep Master Mix Set for 454 contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressio…

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NEBNext® dsDNA Fragmentase Reaction Buffer

New England Biolabs Inc.

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction.NEBNext® dsDNA Fragmentase Reaction Buffer Components: 20mM Tris…

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NEBNext® mRNA Library Prep Master Mix Set for 454™

New England Biolabs Inc.

The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents includ…

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NEBNext® Ultra RNA Library Prep Kit for Illumina®

New England Biolabs Inc.

The NEBNext® Ultra™ RNA Library Prep Kit for Illumina contains enzymes and buffers that are ideally suited for cDNA library preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must p…

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NEBNext® Small RNA Library Prep Set for SOLiD™

New England Biolabs Inc.

The NEBNext Small RNA Library Prep Set for SOLiD™ contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Library Prep Set for Solid is multiplex compatible. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next genera…

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Globin-Zero Gold Kit

Cambio Ltd

The Globin-Zero™ Gold Kit removes the highly abundant globin mRNA and rRNA from human/mouse/rat total blood RNA samples for RNA-Seq sample prep. Removal of these RNA enables deeper sequencing into blood RNA samples.

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ScriptSeq™ Complete Kits

Cambio Ltd

The new ScriptSeq™ Complete Kits combine Epicentre's powerful Ribo-Zero™ technology and rapid ScriptSeq v2 RNA-Seq Library Preparation Kit for seamless, end-to-end preparation of RNA-Seq libraries in 1 day. The ScriptSeq Complete Kits require 1 µg to 5 µg of total RNA and the ScriptSeq™ Complete Kits Low Input require 100 ng to 1 µg of total RNA.

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GS FLX Titanium PicoTiterPlate Kit 70x75

Roche

Kit for determining the nucleotide sequence of an immobilized and clonally amplified DNA library prepared using the appropriate GS FLX Titanium series kits. The GS FLX Titanium PicoTiterPlate Kit 70x75 contains accessories sufficient for one sequencing run on a Genome Sequencer FLX Instrument. The kit is designed for use in combination with the GS FLX Titanium Sequencing Kit XLR70.Contents 70x75 GS FLX Titanium PTP Device 70x…

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GS FLX Titanium Control Bead Kit

Roche

The GS FLX Titanium Control Bead Kit can be used as a standard sequencing control on the Genome Sequencer FLX Instrument. During a test sequencing Run, only Control Beads are sequenced. The Control Beads contain known DNA sequences that should yield sequencing results within expected parameters if the instrument is performing to specifications. The GS FLX Titanium Control Bead Kit is used in combination with a GS FLX Titanium…

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GS FLX Titanium Sequencing Kit XLR70

Roche

For use with the GS FLX Titanium PicoTiterPlate Kit 70x75 and the Genome Sequencer FLX Instrument. The GS FLX Titanium Sequencing Kit XLR70, combined with the GS FLX Titanium PicoTiterPlate Kit 70x75, contains all reagents and other components required for one sequencing run on a Genome Sequencer FLX Instrument. The kit is designed for use with the GS FLX Titanium PicoTiterPlate Kit 70x75 and the Genome Sequencer FLX Instrume…

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