Next Generation Sequencing Products & Reviews

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Next-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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NGS Custom Panels

Twist Bioscience

Twist Custom Panels can be designed and built to cover a wide range of panel sizes, target regions, and multiplexing requirements — all with exceptional and consistent performance. Whether you design your own panel from scratch or add targets to enhance the content of existing panels, you can use your Twist Custom Panel with Twist’s modular library preparation kits or seamlessly integrate them into an existing workflow.

4.7/5.0
|6 Reviews
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Twist Human Core Exome Kit

Twist Bioscience

Twist Bioscience, the leader in synthetic DNA with unparalleled precision at scale, is redefining targeted sequencing performance with the Twist Human Core Exome Kit. This kit includes library preparation and enrichment components, allowing the flexibility to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

4.7/5.0
|3 Reviews
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Twist Comprehensive Exome Panel

Twist Bioscience

Exome sequencing has become a widely used practice in clinics and diagnostics. The superior performance of the Twist Human Core Exome provides the optimal solution for sequencing of protein coding genes, while focusing on the most accurate curated subset— CCDS database.

4.9/5.0
|2 Reviews
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Twist Synthetic SARS-CoV-2 RNA Controls

Twist Bioscience

Positive controls provide quality control measures for the verification and validation for point-of-care diagnostic tests including both next-generation sequencing (NGS) and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Twist offers fully synthetic SARS-CoV-2 RNA controls, available for dozens of distinct reference sequences.

4.9/5.0
|5 Reviews

Twist Library Preparation EF 2.0 Kit (Enzymatic Fragmentation)

Twist Bioscience

Twist Library Preparation Enzymatic Fragmentation (EF) Kit 2.0 is built to help you achieve a more efficient sequencing pipeline and obtain more accurate Next-Generation Sequencing (NGS) results. This updated kit has new fragmentation and ligation modules, and includes the Equinox Library Prep Amp Mix. This hot-start enzyme formulation has a lower error rate* and high efficiency at low input volumes—making it ideal for samples…

4.9/5.0
|2 Reviews
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Custom SureSelect

Agilent Technologies

Agilent’s market leading SureSelect platform provides a complete portfolio of catalog and custom products, providing the flexibility you need from discovery to follow-up studies. Agilent’s custom solutions allow you to target your regions of interest using our free web portal SureDesign. Custom solutions are available for DNA and RNA target enrichment.

5.0/5.0
|1 Review

SureSelect Target Enrichment System Kit

Agilent Technologies

Agilent's SureSelect market leading platform provides a complete portfolio of catalog and custom products, providing the flexibility you need, for your discovery to follow-up studies. Agilent™s SureSelect Target Enrichment System is a highly efficient hybrid selection technique for optimizing next-generation sequencing. It is available for the Illumina GA and HiSeq, Applied Biosystems SOLiD, and 454 Life Sciences FLX and GS J…

4.7/5.0
|1 Review

Fosmid Library

Bio S&T

Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Also, the copy control vector can be used for easy DNA purification from fosmid clones. Fosmid libraries are commonly used for closing gaps in a whole genome se…

5.0/5.0
|1 Review

MiSeq Personal Sequencer

Illumina

Illumina's MiSeq is the only fully integrated personal sequencer, delivering a streamlined solution that takes you from rapid sample prep through automated data analysis and storage in the BaseSpace cloud. Sequencing doesn’t get any easier than this. As the only personal sequencer capable of producing paired-end reads, Illumina's MiSeq system puts the largest portfolio of sequencing applications at your fingertips. Optimized…

4.5/5.0
|28 Reviews



NEBNext® Reagents for DNA Library Preparation

New England Biolabs Inc.

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…

4.7/5.0
|4 Reviews

NEBNext® DNA Library Prep Master Mix Set for Illumina®

New England Biolabs Inc.

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…

5.0/5.0
|1 Review

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

New England Biolabs Inc.

he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.NEBNext® reagents are a series of highly pure reagents that facilitate samp…

4.7/5.0
|2 Reviews

NEBNext® dsDNA Fragmentase®

New England Biolabs Inc.

NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…

4.9/5.0
|2 Reviews

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

New England Biolabs Inc.

The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents…

5.0/5.0
|1 Review

MiSeqDx System

Illumina

The MiSeqDx instrument is the first and only FDA-cleared in vitro diagnostic (IVD) next-generation sequencing (NGS) system. Designed specifically for the clinical laboratory environment, the MiSeqDx instrument offers a small, approximately 4 square feet (0.37 square meters) footprint, an easy-to-follow workflow, and data output tailored to the needs of clinical labs. In addition, the integrated software enables sample trackin…

4.9/5.0
|8 Reviews

NGS STARlet - Automated NGS Library Preparation

Hamilton Company

The NGS STARlet is a compact, flexible and future-proof workstation for library preparation for all common sequencing platforms. It is excellently suited for any throughput from 8 to 48 samples and reduces time consuming manual pipetting steps and sample-to-sample variability. The open workstation concept allows automation of any library preparation chemistry. Already included are validated methods for the NEBNext® Ultra DNA l…

4.9/5.0
|2 Reviews

Ovation® RNA-Seq System V2

NuGEN

Our latest generation Ovation RNA-Seq System V2 provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications, now with enhanced transcript coverage and uniform distribution of sequencing reads. Amplification is initiated at the 3' end as well as randomly throughout the whole transcriptome in the sample. This feature makes the Ovation RNA-Seq System V2 ideal for amplification prior to…

4.9/5.0
|2 Reviews