UHPLC and HPLC Products & Reviews

Size-based separations of peptides, proteins, and viral vectors

XBridge® and ACQUITY UPLC® Oligonucleotide C18 Columns for Synthetic Oligonucleotide Analysis and PurificationIon-pair, reversed-phase separation of detritylated oligonucleotides on sub-3 micron particles yields exceptional component resolution.UPLC ® technology increases sample throughput while maintaining necessary oligonucleotide component resolution.LC/UV and LC/MS methods for enhanced quantitative and qualitative oligonuc…

Spark Holland has been operating at the forefront of autosampler design for 25 years. Spark’s Reliance™ autosampler is the first true bio-injector, and is preferred by customers for 4 simple reasons: -   Lowest possible carry-over-   High-speed wash cycles-   Low maintenance-   Conditioned sample storage The Reliance™ autosampler complies with GLP requirements and can be coupled with most LC-MS systems. It runs fully integra…

Hypersil Green columns allows for sharp, symmetrical and easily quantifiable assays to be developed quickly and easily. Product detail: Hypersil Green columns are developed for environmental analysis. The chromatographic separation of phenols, nitrophenols, chlorophenols, triazines, urea pesticides, phthalates and many other polar compounds requires a separating phase specially designed for the task. Hypersil Green ENV has…

o   The benchmark in based deactivated silica columnso   Sharp peaks and improved symmetry for basic compoundso   Value and performance from a globally referenced column Product detail: DASH columns are packed to the same high standards as our analytical columns, and offer superioir chromatographic resolution in a design that eliminates the need for endfittings and wrenches during column change over. This makes column inst…

Fluophase RP Columns o   A range of perfluorinated phases for alternative selectivityo   Extra retention and selectivity for halogenated compoundso   Select from 100Å and 300Å pore sizes Product detail:For alternative selectivity to traditional alkyl phases, we recommend you try the perfluorinated phases of Fluophase columns. The stationary phase of Fluophase RP contains a perfluorinated C6 chain, where every hydrogen atom on…

Hypersil 100 C8 Columns Compared to other classical materials, Hypersil 100 has a smaller pore diameter of only 100Å, which creates a higher specific surface area that can be advantageous where longer retention times are needed, such as chromatography of polar compounds. Product detail: Compared to other classical materials, Hypersil 100 has a smaller pore diameter of only 100Å, which creates a higher specific surface area…

The UNIGUARD drop-in cartridge fits both our guard systems: the stand alone UNIGUARD and the Direct Connection UNIGUARD. Product detail: We recommend all analytical columns are protected by a guard cartridge, which is replaced on a regular basis. The ID of the analytical column determines the correct ID for the guard cartridge. We offer 10x1.0, 10x2.1, 10x3.0, 10x4.0 (for use with both 4.0 and 4.6mm ID analytical columns) i…

RP-HPLC for Protein/Peptide Analysis and PurificationJupiter® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification. A wide variety of phases (C18, C12, C5, C4) and dimensions (capillary-preparative)/bulk media are available to support the majority of separation requirements.Jupiter 300 - 300 Å columns designed to purify intact proteinsJuptier Proteo - 90 Å columns eng…

Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acid…

Waters ACQUITY UPLC - Dramatic Improvements in Speed, Sensitivity and Resolution Whether you work in methods development or quality assurance, in drug discovery and development or testing for safety in food supplies, you seek a productivity edge. With the ACQUITY UPLC® System, now you can get more information in a single, short run than you've ever seen with HPLC. Waters ACQUITY UPLC System UltraPerformance LC® (UPLC®) techn…

Excellent low pH stability and high mass loading capability

XBridge Columns include a selection of 10 general-purpose and application-specific sorbents that cover a wide range of particles sizes for analytical and preparative HPLC applications.   XBridge Column users can quickly develop robust methods using mobile phases throughout the entire pH range of 1-12. XBridge OBD™ Preparative Column users, benefit from high pH and temperature stability to provide increased mass loading f…

VanGuard Pre-Columns for UPLC® separations are guard columns that are designed for use with the ACQUITY UPLC® system. These guard columns use an ultra-low volume design (patent pending) that efficiently protects UPLC column performance. This ultra-low volume design does not compromise the UPLC holistic design approach to higher efficiency, greater resolution and increased throughput. Guard columns are typically used in appli…

Designed specifically for polymer characterization, the Styragel® columns are grouped into the HR series for low-to-mid molecular weight samples, the HT series for high-temperature applications, and the HMW series for high molecular weight samples. Specifically controlled styrene divinylbenzene formulations provide reproducible performance in your GPC applications.

Packed with hydroxylated polymethacrylate-based gel, Waters Ultrahydrogel™ SEC columns are ideal for the analysis of aqueous-soluble samples, such as oligomers; oligosaccharides; polysaccharides; and cationic, anionic, and amphoteric polymers. Measuring 7.8 mm x 300 mm, these high-resolution columns offer many advantages over conventional aqueous SEC columns, such as: - A wide pH range (2–12) - Compatibility with high concen…

The SPECTRUM range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, in Discovery and Development, who are looking purify samples from mgs to 5-10 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…

The Shimadzu Prominence high-performance liquid chromatograph achieves an exceptional level of performance, reliability and great expandability to support diverse applications from ultra-fast liquid chromatography to preparative LC, gel permeation chromatography (GPC), ion chromatography, and LC/MS.Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to…

The Prominence UFLC Series is an ultra-high-speed LC that achieves both ultra-high-speed analysis and ultra-high separation, based on high analysis precision and reliability. In addition to shortening analysis times, thereby heightening analysis efficiency and conserving solvent, this instrument supports reliable separation and detection of trace materials in a variety of fields. Applications include the evaluation of trace re…

Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) is the technique used for obtaining a rapid and reliable characterization of polymer molecular weight and molecular weight distribution. Clarity Chromatography Software with GPC Extension provides interactive and automated GPC analysis, including recalibration and GPC reporting, as well as simplifies the retrieval of GPC data. The GPC Extension allows fl…

Eliminates the time-consuming, one-at-a-time syringe filter process by simultaneously filtering up to 24 samples in seconds. Reduces labour time by twenty minutes per carousel over the conventional syringe filter technique. Can be used with any conventional 12 x 32 mm vials; simply transfer your filtered samples from the Waters Alliances sample carousel* to any HPLC sample carousel that houses 12 x 32 mm vials and save b…

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase,…

Economical, high efficiency columns to purify and/or characterize proteins, peptides, and other biomolecules including antibodies, aggregates, and PEGylated proteinsHigh performance: Analytical and preparative BioSep columns offer high resolution, maximum efficiency, and exceptional peak asymmetryImproved recovery: Extremely inert media offers advantages for accurte quantitationFlexible method development: 3 phases options (20…

The Prominence UFLC Series is an ultra-high-speed LC that achieves both ultra-high-speed analysis and ultra-high separation, based on high analysis precision and reliability. Utilizing features of the Prominence UFLC, which offered high speed as well as high analysis accuracy and reliability, thereby increasing analysis cost-efficiency, we have developed the Prominence UFLCXR, which achieves greater data quality by means of…

The Surveyor Plus family of HPLC instruments is specifically optimized for the most demanding HPLC and LC/MS applications. Short cycle times, high sample capacity, low flow rate performance, and patented LightPipeTM technology have been integrated into one complete system for high-throughput sample processing environments. The versatility of the modular design, featuring custom quaternary pumping solutions, provides flexibili…