High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
600 bar; superficially porous, 2.7 µm particle6; available in 2.1 - 4.6 mm id; 30 - 150 mm lengths. ZORBAX Eclipse Plus columns feature improved silica, special bonding and optimized endcapping that combine to deliver exceptional peak shapes for a wide range of analytes. Agilent's Poroshell 120 columns have a solid 1.7 µm core and a porous .5 µm outer layer, for a total 2.7 µm particle that delivers high resolution and high…
600 bar; superficially porous, 2.7 µm particle6; available in 2.1 - 4.6 mm id; 30 - 150 mm lengths. ZORBAX Eclipse Plus columns feature improved silica, special bonding and optimized endcapping that combine to deliver exceptional peak shapes for a wide range of analytes. Agilent's Poroshell 120 columns have a solid 1.7 µm core and a porous .5 µm outer layer, for a total 2.7 µm particle that delivers high resolution and high…
400 bar; available in 2.1 - 4.6 mm id; 30, 50, 75, 100, 150 mm lengths. Manufactured by Agilent to rigorous QC specifications, the ZORBAX family encompasses 5 particle sizes and more than 32 phases, for reliable scalability and transferability from HPLC to UHPLC, from lab to lab and around the world.
Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5.
Experience the power of analytical compound isolation
The 1260 Infinity II LC System is a robust HPLC that offers the widest choice of modules for analytical HPLC and entry-level UHPLC, delivering the performance, reliability, and robustness you need for highest confidence in your daily results.
The 1260 Infinity II Bio-Inert Pump is a quaternary UHPLC pump that is made of bio-inert material for use in biological and extreme pH applications. This pump enables gradient formation and solvent blending from up to four solvents and operates at pressures up to 600 bar and flow rates up to 10 mL/min.
The 1290 Infinity II Diode Array Detector (DAD) delivers multiple wavelength detection with full spectra at sampling rates up to 240 Hz for ultrafast UHPLC separations. The Max-Light cartridge cell features optofluidic waveguides, which improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects.
The 1260 Infinity II Preparative Autosampler is designed for automated sample handling in routine purification applications. This flow-through vialsampler supports preparative flow rates of 200 mL/min and 600 bar pressure. Increase your daily throughput of purified sample by injecting up to 132 preparative samples with a maximum of 3.6 mL volume.
