High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Acetonitrile for use with HPLC by Rankem ®
Monolithic silica-based Chromolith® HPLC columns allow rapid separations at very low column backpressure with high matrix tolerance and extended column lifetime, thus making them ideal products for high throughput and cost-efficient analyses of matrix-rich samples.
Cannabis potency testing is performed using HPLC. Acetonitrile from Spectrum Chemical exceeds ACS Specifications and HPLC Grade as a polar aprotic solvent in organic synthesis as well as a carrier (mobile phase) solvent in HPLC. Acetonitrile is a medium-polarity solvent with a convenient liquid range that is miscible with water, making it one of the most popular HPLC solvent choices.
Flexibility, performance and efficiency with the PerkinElmer LC 300 HPLC and UHPLC System
Develosil HPLC columns provide high efficiency, high resolution separations.
Designed to meet the demands of modern, large-scale manufacturers Capto Q’s shorter processing times can reduce exposure of the target protein.
Spark Holland has been operating at the forefront of autosampler design for 25 years. Spark’s Reliance™ autosampler is the first true bio-injector, and is preferred by customers for 4 simple reasons: - Lowest possible carry-over- High-speed wash cycles- Low maintenance- Conditioned sample storage The Reliance™ autosampler complies with GLP requirements and can be coupled with most LC-MS systems. It runs fully integra…
Hypersil Green columns allows for sharp, symmetrical and easily quantifiable assays to be developed quickly and easily. Product detail: Hypersil Green columns are developed for environmental analysis. The chromatographic separation of phenols, nitrophenols, chlorophenols, triazines, urea pesticides, phthalates and many other polar compounds requires a separating phase specially designed for the task. Hypersil Green ENV has…
o The benchmark in based deactivated silica columnso Sharp peaks and improved symmetry for basic compoundso Value and performance from a globally referenced column Product detail: DASH columns are packed to the same high standards as our analytical columns, and offer superioir chromatographic resolution in a design that eliminates the need for endfittings and wrenches during column change over. This makes column inst…
Fluophase RP Columns o A range of perfluorinated phases for alternative selectivityo Extra retention and selectivity for halogenated compoundso Select from 100Å and 300Å pore sizes Product detail:For alternative selectivity to traditional alkyl phases, we recommend you try the perfluorinated phases of Fluophase columns. The stationary phase of Fluophase RP contains a perfluorinated C6 chain, where every hydrogen atom on…
Hypersil 100 C8 Columns Compared to other classical materials, Hypersil 100 has a smaller pore diameter of only 100Å, which creates a higher specific surface area that can be advantageous where longer retention times are needed, such as chromatography of polar compounds. Product detail: Compared to other classical materials, Hypersil 100 has a smaller pore diameter of only 100Å, which creates a higher specific surface area…
The UNIGUARD drop-in cartridge fits both our guard systems: the stand alone UNIGUARD and the Direct Connection UNIGUARD. Product detail: We recommend all analytical columns are protected by a guard cartridge, which is replaced on a regular basis. The ID of the analytical column determines the correct ID for the guard cartridge. We offer 10x1.0, 10x2.1, 10x3.0, 10x4.0 (for use with both 4.0 and 4.6mm ID analytical columns) i…
RP-HPLC for Protein/Peptide Analysis and PurificationJupiter® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification. A wide variety of phases (C18, C12, C5, C4) and dimensions (capillary-preparative)/bulk media are available to support the majority of separation requirements.Jupiter 300 - 300 Å columns designed to purify intact proteinsJuptier Proteo - 90 Å columns eng…
Setting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acid…
The SPECTRUM range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, in Discovery and Development, who are looking purify samples from mgs to 5-10 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…
The Shimadzu Prominence high-performance liquid chromatograph achieves an exceptional level of performance, reliability and great expandability to support diverse applications from ultra-fast liquid chromatography to preparative LC, gel permeation chromatography (GPC), ion chromatography, and LC/MS.Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to…
The Prominence UFLC Series is an ultra-high-speed LC that achieves both ultra-high-speed analysis and ultra-high separation, based on high analysis precision and reliability. In addition to shortening analysis times, thereby heightening analysis efficiency and conserving solvent, this instrument supports reliable separation and detection of trace materials in a variety of fields. Applications include the evaluation of trace re…
Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) is the technique used for obtaining a rapid and reliable characterization of polymer molecular weight and molecular weight distribution. Clarity Chromatography Software with GPC Extension provides interactive and automated GPC analysis, including recalibration and GPC reporting, as well as simplifies the retrieval of GPC data. The GPC Extension allows fl…
Eliminates the time-consuming, one-at-a-time syringe filter process by simultaneously filtering up to 24 samples in seconds. Reduces labour time by twenty minutes per carousel over the conventional syringe filter technique. Can be used with any conventional 12 x 32 mm vials; simply transfer your filtered samples from the Waters Alliances sample carousel* to any HPLC sample carousel that houses 12 x 32 mm vials and save b…
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase,…
ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds. What is HILIC? HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents. In HILIC one has: A c…
Economical, high efficiency columns to purify and/or characterize proteins, peptides, and other biomolecules including antibodies, aggregates, and PEGylated proteinsHigh performance: Analytical and preparative BioSep columns offer high resolution, maximum efficiency, and exceptional peak asymmetryImproved recovery: Extremely inert media offers advantages for accurte quantitationFlexible method development: 3 phases options (20…
The Prominence UFLC Series is an ultra-high-speed LC that achieves both ultra-high-speed analysis and ultra-high separation, based on high analysis precision and reliability. Utilizing features of the Prominence UFLC, which offered high speed as well as high analysis accuracy and reliability, thereby increasing analysis cost-efficiency, we have developed the Prominence UFLCXR, which achieves greater data quality by means of…
Designed to meet the challenges of today's laboratory environment, fast results and demanding workload, LaChrom Elite® couples automated tasks with innovative design. Control by Empower™ 2 software facilitates regulatory compliance Features Innovation • System synchronization, Pump-Autosampler Synchronization (PASS) This unique feature means that each injection occurs at exactly the same point in every pump cycle ensuring the…
