Gene Expression and Molecular Cloning Products & Reviews

Single guide RNA expressing vectors for effective and accurate gene knockout

Combine sgRNA and Cas9 nuclease expression into a single vector for simplified delivery

Our CRISPR Design Tool enables an intuitive one-stop location for guide RNA design and ordering. Utilize the design tool to order guide RNAs for targeted gene knockout or location-specific HDR-mediated genome editing.

Optimize your CRISPR-Cas9 experiments with our specific controls and primers

A direct and efficient set of tools to study a gene’s function by overexpression in its native context

Baculovirus cassette vector pAc-kappa-CH3 for the expression of human, humanized or chimeric IgG (kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments.

The safe alternative to using live coronavirus

The safe alternative to using live coronavirus

Direct translocation of proteins into living cells

Pre made lentiviral particles for flourescent proteins

Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.

With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment.

For transient gene repression with CRISPR-level specificity

Horizon's CRISPRi guide RNA in an easy to deliver lentiviral format

Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in mRNA format

Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in lentiviral particle format

A direct and efficient set of tools to study a gene’s function by transcriptional interference in its native context

SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).

25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water

100 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water  

500 x 20 µl reactions; bundle of 5 boxes of 100 reaction kit; includes 2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water  

Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi.…

A push-button, benchtop biofoundry

Enables the direct capture and quantitation of different serotypes of AAV in crude lysates, column eluates, cell lysates and cell culture supernatants using the CaptureSelect™ anti-AAVX ligand. Total AAV capsid is determined by this quantitation technique.

GMP-manufactured Cas9 protein for clinical research