Gene Expression and Molecular Cloning Products & Reviews

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Hybridizes to crRNA to activate the Cas9 enzyme.

Hybridizes to crRNA to activate the Cas9 enzyme.

urified carrier DNA that improves delivery of Cas9 ribonucleoprotein (RNP) by electroporation.

Purified carrier DNA that is required for efficient delivery of Cpf1 ribonucleoprotein (RNP) by electroporation.

For detection of on-target or known off-target CRISPR events in cultured cells

An advanced data analysis pipeline for quantifying your genome editing results

A small molecule compound that has demonstrated an ability to increase the rate of homology-directed repair (HDR).

Deep, targeted amplicon sequencing with highly multiplexed panels for sequencing on Illumina platforms.

Complete synthetic genes with 100% sequence verification are provided in a plasmid cloning vector and ready to use in a variety of applications.

Double-stranded DNA fragments for quick and cost-effective cloning

Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.

With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment.

For transient gene repression with CRISPR-level specificity

Horizon's CRISPRi guide RNA in an easy to deliver lentiviral format

Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in mRNA format

Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in lentiviral particle format

A direct and efficient set of tools to study a gene’s function by transcriptional interference in its native context

SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).

25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water

100 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water  

500 x 20 µl reactions; bundle of 5 boxes of 100 reaction kit; includes 2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water  

Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi.…

A push-button, benchtop biofoundry

Enables the direct capture and quantitation of different serotypes of AAV in crude lysates, column eluates, cell lysates and cell culture supernatants using the CaptureSelect™ anti-AAVX ligand. Total AAV capsid is determined by this quantitation technique.