Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Gibson Assembly Ultra kit builds on Codex DNA’s original Gibson Assembly ® technology to expand the ranges of fragment size, fragment number, and project complexity that are compatible with scarless and high-fidelity cloning.
The Gibson Assembly ® HiFi kit uses the original Gibson Assembly ® cloning* method, which has been optimized for the seamless joining of up to five inserts into a plasmid vector, resulting in DNA constructs with reliably low error rates.
Synthetic single guide RNAs for efficient gene knockout & unparalleled specificity
Synthetic guide RNAs for efficient gene knockout & unparalleled specificity
Single guide RNA expressing vectors for effective and accurate gene knockout
Combine sgRNA and Cas9 nuclease expression into a single vector for simplified delivery
Our CRISPR Design Tool enables an intuitive one-stop location for guide RNA design and ordering. Utilize the design tool to order guide RNAs for targeted gene knockout or location-specific HDR-mediated genome editing.
Optimize your CRISPR-Cas9 experiments with our specific controls and primers
A direct and efficient set of tools to study a gene’s function by overexpression in its native context
Baculovirus cassette vector pAc-kappa-CH3 for the expression of human, humanized or chimeric IgG (kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments.
Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.
With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment.
For transient gene repression with CRISPR-level specificity
Horizon's CRISPRi guide RNA in an easy to deliver lentiviral format
Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in lentiviral particle format
A direct and efficient set of tools to study a gene’s function by transcriptional interference in its native context
SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).
Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi.…
Enables the direct capture and quantitation of different serotypes of AAV in crude lysates, column eluates, cell lysates and cell culture supernatants using the CaptureSelect™ anti-AAVX ligand. Total AAV capsid is determined by this quantitation technique.
GMP-manufactured Cas9 protein for clinical research
Next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency
ClonExpress ® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector.
