UHPLC and HPLC Products & Reviews
High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
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Pocket Surveyor
Thermo Fisher ScientificThe new Finnigan Pocket Surveyor software providing local instrument control for new and existing Finnigan Surveyor HPLC Systems. The software operates on a handheld computer running the popular Windows Pocket PC 2002 O/S and offers chromatographers the ability to connect directly to their Surveyor quaternary gradient pump and/or variable wavelength detector. In addition to individual module control, the Finnigan Pocket Sur…
Thermo Scientific™ Acclaim™ Trinity P1 LC Column
Thermo Fisher ScientificAcclaim™ Trinity P1 is a unique, high-efficiency, silica-based column designed for pharmaceutical applications such as simultaneous separation of pharmaceutical drug substances and their counterions. his new column, based on nanopolymer silica hybrid (NSH) technology, provides multiple retention mechanisms including reversed-phase, anion-exchange, and cation-exchange. As a result, its selectivity can be easily opti…
Thermo Scientific™ Acclaim™ Explosives LC Column
Thermo Fisher ScientificThe Thermo Scientific Acclaim™ Explosives E1 (5 µm) and E2 (5, 3 and 2.2 μm) silica-based reversed-phase columns are designed to resolve all 14 explosives listed in EPA SW-846 Method 8330: Nitroaromatics and Nitramines by HPLC. Their unique chemistries deliver superior resolution with complementary selectivities. The E1 is recommended for use as a direct replacement for ODS columns for the primary analysis.The E2 may be used a…
Thermo Scientific™ Acclaim™ 300 C18 Protein and Peptide Column
Thermo Fisher ScientificHigh-Resolution Separation of Proteins and Peptides The Acclaim® 300 features 3 µm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residua…
Thermo Scientific™ DNASwift™ SAX-1S Oligonucleotide Columns
Thermo Fisher ScientificThe DNASwift™ SAX-1S is a strong anion-exchange column that provides exceptionally high resolution for the purification of oligonucleotides.The exceptionally high resolution provides superior purity and yields compared to other ion-exchange or reversed-phase columns available. The DNASwift column is ideal for therapeutic and diagnostic research, which have very high purity and yield oligonucleotides purification requirements.T…
Thermo Scientific™ Acclaim™ PepMap™ 100 C18 Protein and Peptide Column
Thermo Fisher ScientificThe Thermo Scientific Acclaim™ PepMap™ 100 C18 column sets the benchmark in peptide separation.The PepMap100 C18 column is available in 3 or 5 µm particle sizes, with 100 Å pore sizes, and in nano, capillary, and micro formats. nanoViper fingertight fittings allow for easy column installation.Column Details: Chemistry: C18 Particle Size: 3, 5 μm Pore Size: 100 Å Length: 5, 15, 25, 50 mm ID: 75, 300 μm, 1.0 mm
Thermo Scientific™ Acclaim™ PepMap300 C18 Protein and Peptide Column
Thermo Fisher ScientificAcclaim™ PepMap300 C18 is used for the separation of larger peptides and proteins.It is available in 5 µm particle sizes, with 300 Å pore sizes, and in nano, capillary, and micro formats.Column Details: Chemistry: C18 Particle Size: 3, 5 μm Pore Size: 100, 300 Å Length: 5, 15 mm ID: 75, 300 μm, 1 mm
Thermo Scientific™ MAbPac™ SCX-10 Monoclonal Antibody Analysis Column
Thermo Fisher ScientificMAbPac™ SCX-10 Columns for the High-Resolution Separation and Analysis of Monoclonal Antibody VariantsThe Thermo Scientific™ MAbPac™ SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with…
Thermo Scientific™ ProPac™ SAX and WAX Protein Column
Thermo Fisher ScientificHigh-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of ProteinsThe ProPac® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anio…
Thermo Scientific™ ProPac™ SCX and WCX Protein Column
Thermo Fisher ScientificStrong and Weak Cation-Exchange Columns for High-Resolution Separation of Proteins and MAbsThe Thermo Scientific ProPac™ SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resoluti…
Thermo Scientific™ ProPac™ PA1 Protein and Peptide Column
Thermo Fisher ScientificFor Hydrophilic Anionic Protein SeparationsThe ProPac® PA1 column supports the analysis and purification of hydrophilic anionic proteins and peptides. Proteins and peptides that are highly cationic (e.g., those with very high isoelectric points) may be resolved on this column, but some may exhibit substantial binding to the column.ProPac® PA1 Protein and Peptide Column Features: Good for hydrophilic anionic proteins and pepti…
Thermo Scientific™ ProPac™ HIC Protein and Peptide Column
Thermo Fisher ScientificHydrophobic Interaction Chromatography Columns for the Separation of Large BiomoleculesHydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.The ProPac® HIC-10 column is a high-re…
Thermo Scientific™ ProSwift™ ConA-1S Affinty Column
Thermo Fisher ScientificProSwift™ ConA-1S Affinity Column for Glycan, Glycopeptide and Glycoprotein Enrichment and Purification The ProSwift ConA-1S affinity monolith column is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins from complex samples.The HPLC- compatible ProSwift ConA-1S affinity column maintains the specificity and selectivi…
Thermo Scientific™ ProSwift™ RP Protein Column
Thermo Fisher ScientificMonolith Reversed-Phase Columns for High-Resolution Protein Separations at High Flow Rates ProSwift® reversed-phase monolith columns uniquely provide the advantages of high resolution at exceptionally high flow rates for protein separations. They deliver the outstanding resolving power of nonporous analytical media combined with faster separations than any bead based columns available. ProSwift polymer monolith columns achieve…
Thermo Scientific™ ProSwift™ IEX Protein Column
Thermo Fisher ScientificMonolith Ion-Exchange Columns for Superior High-Resolution Analytical and Preparative Protein Separations The ProSwift SCX monolith column line has been expanded to include a new 1 x 50 mm column. As with all ProSwift monoliths, the fast mass transfer allows for fast protein separations. ProSwift monoliths offer excellent separations using fast gradients at low and high flow rates, which improve productivity.ProSwift Monolith…
HyPURITY AQUASTAR Columns
Thermo Fisher ScientificA new member of the HyPURITY® family introduced at Pittcon 2003, HyPURITY AQUASTAR™ columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. Product detail:A new member of the HyPURITY® family introduced at Pittcon 2003, HyPURITY AQUASTAR™ columns are a new silica-based polar end-capped C18 which is designed to off…
Ion Exchange Columns
Thermo Fisher ScientificBioBasic AX Columns o Improved reproducibility, efficiency and column lifetimeso Range of stationary phases to optimize selectivityo Powerful resolution, even on short column lengths Product detail: New BioBasic AX (weak anion exchange) columns have a polyethyleneimine (PEI) ion exchange group and a polymer coating, specifically designed to give robust and clean performance in ion exchange of acidic proteins and pep…
Thermo Scientific™ Acclaim™ C30 Reversed-Phase LC Columns
Thermo Fisher ScientificThe Acclaim™ C30 is designed to provide high shape selectivity for separation of hydrophobic structurally related isomers and unique selectivity complementary to other reversed-phase columns. Acclaim C30 columns are available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 200 Å. It is based on covalent modification of C30 alkyl silane on high-purity, spherical, poro…
Transgenomic WAVE® System
Transgenomic Inc.The Transgenomic WAVE® System is among the most sensitive and accurate technologies for detection of unknown genetic mutations and single nucleotide polymorphisms (SNPs).
WaveQuest HPLC Detector
Cecil Instruments LimitedPerform detailed spectral scans and highly sensitive quantitative analyses on the same instrument.