UHPLC and HPLC Products & Reviews

High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Scientific™ ProPac™ PA1 Protein and Peptide Column

Thermo Fisher Scientific

For Hydrophilic Anionic Protein SeparationsThe ProPac® PA1 column supports the analysis and purification of hydrophilic anionic proteins and peptides. Proteins and peptides that are highly cationic (e.g., those with very high isoelectric points) may be resolved on this column, but some may exhibit substantial binding to the column.ProPac® PA1 Protein and Peptide Column Features: Good for hydrophilic anionic proteins and pepti…

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Thermo Scientific™ ProPac™ HIC Protein and Peptide Column

Thermo Fisher Scientific

Hydrophobic Interaction Chromatography Columns for the Separation of Large BiomoleculesHydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.The ProPac® HIC-10 column is a high-re…

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Thermo Scientific™ ProSwift™ ConA-1S Affinty Column

Thermo Fisher Scientific

ProSwift™ ConA-1S Affinity Column for Glycan, Glycopeptide and Glycoprotein Enrichment and Purification The ProSwift ConA-1S affinity monolith column is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins from complex samples.The HPLC- compatible ProSwift ConA-1S affinity column maintains the specificity and selectivi…

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Thermo Scientific™ ProSwift™ RP Protein Column

Thermo Fisher Scientific

Monolith Reversed-Phase Columns for High-Resolution Protein Separations at High Flow Rates ProSwift® reversed-phase monolith columns uniquely provide the advantages of high resolution at exceptionally high flow rates for protein separations. They deliver the outstanding resolving power of nonporous analytical media combined with faster separations than any bead based columns available. ProSwift polymer monolith columns achieve…

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Thermo Scientific™ ProSwift™ IEX Protein Column

Thermo Fisher Scientific

Monolith Ion-Exchange Columns for Superior High-Resolution Analytical and Preparative Protein Separations The ProSwift SCX monolith column line has been expanded to include a new 1 x 50 mm column. As with all ProSwift monoliths, the fast mass transfer allows for fast protein separations. ProSwift monoliths offer excellent separations using fast gradients at low and high flow rates, which improve productivity.ProSwift Monolith…

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Thermo Scientific™ Acclaim™ C30 Reversed-Phase LC Columns

Thermo Fisher Scientific

The Acclaim™ C30 is designed to provide high shape selectivity for separation of hydrophobic structurally related isomers and unique selectivity complementary to other reversed-phase columns. Acclaim C30 columns are available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 200 Å. It is based on covalent modification of C30 alkyl silane on high-purity, spherical, poro…

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Thermo Scientific™ Acclaim™ Trinity P2 LC Column

Thermo Fisher Scientific

The Thermo Scientific™ Acclaim™ Trinity™ P2 column is a high-performance, silica-based column specifically designed for separation of pharmaceutical mono- and multi-valent counterions by high-performance liquid chromatography (HPLC). This column was engineered for analytical chemists who need simple, robust, fast, generic methods. The Acclaim Trinity P2 columns provide an effective solution for counterion analysis using one co…

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Thermo Scientific™ GlycanPac™ AXH-1 HPLC Columns

Thermo Fisher Scientific

Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules.This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologi…

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity LC Columns

Thermo Fisher Scientific

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical da…

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