Genome Analysis Products & Reviews

Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.…

Thermo Scientific Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment…

Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I trea…

Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands.…

Thermo Scientific Verso 1-Step RT-PCR system is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to c…

Thermo Scientific Gene Frame products feature a dual strength adhesive system which facilitates the easy removal of the frames after use. The adhesives on either side of the frame are of differing strengths; the side which adheres to the glass has an easy release adhesive whereas the reverse, which sticks to the coverslip, forms a stronger bond. This means that when the coverslip is removed, due to the disparity in the strengt…

Thermo Scientific Superladders are derived from cloned and purified DNA fragment repeats. A range of sizes are available. Superladder-Low 20bp LadderSize range: 20-1000bpNumber of bands: 50 Superladder-Low 100bp Ladder Size range: 100-1000bp Number of bands: 10Superladder-Mid 100bp Ladder Size range: 100-3000bp Number of bands: 30Superladder-Mid 200bp Ladder Size range: 200-6000bp Number of bands: 30

Thermo Scientific has a wide range of DNA digest markers for low and high molecular weight products. Markers are supplied with loading dye allowing the same loading dye to be used for the sample and the marker. Markers available:PhiX174 RF DNA Hae III MarkerPhiX174 RF DNA Hinc II MarkerPhiX174 RF DNA Hinf I MarkerpBR322 hae III MarkerpBR322 Msp I MarkerLambda Hind III MarkerLambda EcoRI/ Hind III Marker

The Thermo Scientific Dye Terminator Removal Kit is designed to perform the essential removal of unbound fluorescently-labelled dideoxy-ribonucleotides (ddNTPs) and excess salt from sequencing reactions prior to sequence analysis. The kit includes a 96-well plate containing pre-hydrated gel separation matrix consisting of spheres with uniformly sized pores. Sequencing reactions are passed through the separation matrix, which r…

The Thermo Scientific Separation Plate can be used for gel filtration applications (eg. dye terminator removal) when a suitable matrix is added. Features • 96 wells containing polyethylene frits, pore size of 30–45µm • 0.6ml well volume • Ready-to-use microplate format • Suitable for centrifugation

Thermo Scientific Tth DNA Ligase catalyses the ligation between double-stranded DNA molecules with 3’ hydroxyl and 5’ phosphate termini. The enzyme will also repair single-stranded nicks in double-stranded nucleic acids. Features • Ligates double-stranded DNA • Repairs single strand nicks in double-stranded DNA

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers A…

Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…

Thermo Scientific T4 Polynucleotide Kinase (PNK) is a tetrameric protein composed of four identical subunits. It is widely used in molecular biology to radiolabel nucleic acids and to add a phosphate to the 5'-end of dephosphorylated nucleic acids to facilitate ligations. Features • Phosphorylates 5’ ends • Transfers 32P to DNA and RNA

Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.

Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.

Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.

Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.

Thermo Scientific Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5´ phosphates from DNA and RNA and is completely and irreversibly inactivated by heating at 65°C for 15 minutes. SAP is active on 5´ overhangs, 5´ recessed and blunt ends.

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indoyl-ß-D-galactopyranoside) is a chromogenic substrate used to identify recombinant plasmids. In the presence of X-Gal and IPTG, lac+ bacterial colonies appear blue, whereas recombinant colonies appear white.

Built on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt DNAdvance kit is a high throughput genomic DNA (gDNA) isolation reagent kit enabling the purification of high quality DNA from mammalian tissue samples.

Source: UCSC Build: HG18, NCBI 36 Probe Length: 60mer Median Probe Spacing: 1169bp Platform: HD2 Probes per Array: 2,100,000 Feature Size: 13μm x 13μm Array Dimensions: 62mm x 14mm Overall Slide Dimensions: 1" x 3" (25 x 75mm) Recommended Storage: Store arrays desiccated at room temperature.

Kit includes OpenArray® Endpoint Analysis Software CD and user’s manual. Compatible with the NT Imager or NT Cycler.

Customer-owned TaqMan® assays reformatted on OpenArray® plates. Plates must be ordered in multiples of 10. Minimum order is 10 plates. Kit includes: Cases, Immersion Fluid, Case Sealing Glue and Plate-File CD.

OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for ADME and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real…