Polymerase
MedixMDx qPCR Lyo ready mix contains optimized excipients, buffer, PCR enhancers, and an antibody-regulated hot-start Taq DNA polymerase and is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target-specific primers/probes to the master mix, the mixture can be lyophilized directly, without the need to add additional excipients.
MedixMDx qRT-PCR Lyo ready mix is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target specific primers/probes and reverse transcriptase (RTase Lyo) to the mastermix, the mixture can be lyophilized directly, without the need to add additional excipients.
Reliable hot start Taq DNA Polymerase for sensitive detection of low abundance target DNA. Ideal for standard end point PCR and qPCR applications. Available in Glycerol-free, Triton-free, and high concentration formulations.
T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity. Key features: Reliable transcription
Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction with 18-fold increased fidelity compared to Taq DNA Polymerase. High Fidelity PCR…
Taq DNA Polymerase, 1 U/µl can be used instead of the higher concentration of Taq DNA Polymerase for all applications described. The supplied concentration allows accurate and convenient pipetting of small amounts of the polymerase. Benefit Obtain reliable, reproducible results. Roche Applied Science’s Taq DNA Polymerase exemplifies lot-to-lot consistency – tube-to-tube, experiment-to-experiment. Eliminate testing of each…
Thermostable DyNAzyme™ I DNA Polymerase is isolated and purified from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases.DyNAzyme I DNA Polymerase has a half-life of 3 hours at 96 °C. DyNAzyme I DNA Polymerase contains 5’-->3’ exconuclease activity but lacks 3'-->5' proofreading activity. DyNAzyme I DNA Polymerase is p…
Thermostable DyNAzyme™ II DNA Polymerase is isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus which is a Finnzymes proprietary strain. DyNAzyme II DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases. DyNAzyme II DNA Polymerase has a half-life of 2.5 hours at 96 °C. DNA Polymerase contains 5’-->3’ exconuclease ac…
DyNAzymeâ„¢ EXT DNA Polymerase is an optimized mixture of DyNAzymeâ„¢ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization. DyNAzyme EXT Polymerease offers exceptional performance that Taq based mixtures cannot match.
Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers A…
Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…
Top DNA polymerase is a novel thermostable DNA polymerase that is more processive than Taq DNA polymerase. In fact, the extension rate of Top DNA Polymerase is > 3 x that of Taq DNA Polymerase! Top DNA Polymerase can be used for a variety of PCR applications (including TA cloning) and is a robust enzyme for everyday PCR. It contains no proofreading or 5’-3’ Exonuclease activity.Features and Benefits• Fast: More than three tim…
