Oligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.
Glen-Pak™ cartridges utilize the 5’ DMT retained on an oligonucleotide to specifically bind full-length sequences to the support. During the purification process, the failure sequences are eliminated and the DMT is subsequently removed, allowing for elution of purified product.
Effective, stable, non-aqueous oxidizer for DNA synthesis
MGB Eclipse ® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization, while the Eclipse ® has a broad absorption range that quenches many of the most common fluorophores, including FAM, HEX, TET and Yakima Yellow ® .
Multi lingual single beam spectrophotometer, for life sciences measurements. Nano cells may be used
BioAquarius spectrophotometers, pre-programmed for DNA, RNA, protein and life science analyses.
Multi lingual GeneQuest, spectrophotometer for fast, reliable and easy DNA and RNA measurements.
CDP-Star® and CSPD® chemiluminescent substrates for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.
ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Random Decamers are truly random oligo deoxyribonucleotides that can be used to create a pool of cDNAs for subsequent applications, thus providing maximum flexibility.
Thermo Scientific Random Hexamers are truly random oligo deoxyribonucleotides. Hexamers are ideal primers for mRNA with or without a poly-A tail. Hexamers bind more often than decamers, giving higher yields and increased sensitivity when amplifying short fragments.
STN is an online database service that provides global access to published research, journal literature, patents, structures, sequences, properties, and other data.
Specially designed for use with the Cecil Adept HPLC, Q-Adept HPLC, Merit HPLC and IonQuest ion chromatography.
Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…
Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…
Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…
Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…
Phosphoramidites for nucleic acid labeling, microarray labeling, and moreFeatures• Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.• t-butylamine/H2O/MeOH is recommended for cleavage and deprotection steps of TAMRA modified oligos.• dI-phosphoramidite can perform as an universal base as it hybridizes with any of the four bases.Technical Info…
High load CPG prepackaged Universal solid support for DNA SynthesisControlled Pore Glass (CPG) is the gold standard for solid phase oligonucleotide synthesis, with features such as high surface area, tightly controlled pore size and chemical inertness.Our High load CPG for DNA synthesis is available with or without phosphoramidites and can be purchased for standard synthesis sizes or for long-mer synthesis.Features• Utilizes C…
For routine applications, Bioneer offers standard custom oligos with no modification, Bio-RP purification, provided in a tube format. The chart in the ordering information tab shows pricing at various synthesis scales, but please contact your sales representative to learn about any institutional or volume discounts we might have in place for our oligonucleotides.
