ResourceLife Sciences
Applications of a Highly Uniform UV Illumination Imaging System for Quantitative DNA and Protein Analysis
28 Mar 2011This application note by UVP reports on the development and characterisation of a highly uniform UV transillumination system. Quantitative analysis of proteins and nucleic acids is limited by the extreme lack of illumination uniformity across the surface of typical UV light boxes. Here use of a high-density lighting system with a tuned phosphor coating, uniformity of <5% coefficient of variance (CV) across the full imaging surface has been achieved and applied to proteomic analysis.
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Electrophoresis Cell Adhesion Assays Cell / Tissue CultureProteomicsPCR and Thermal Cycling Gel Doc / Image AnalysisAssay AssemblyMedicinal ChemistryCell-Based Assays DNA / RNA Extraction and PurificationDNA Sequencing Protein PurificationGene Expression and Molecular Cloning RNA Interference Biopharmaceutical Advances