Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
The Rac1/Cdc42 Activation Assay provides an effective method for detecting Rac and Cdc42 activity in cell lysates. This assay uses the downstream effector of Rac/Cdc42, p21-activated protein kinase (PAK1), to isolated the active GTP-bound form of Rac/Cdc42 from the sample. The p21 binding domain (PBD) of PAK1 is expressed as a GST-fusion protein and coupled to agarose beads. After precipitation, an immunoblot is performed and…
Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of FPR1 and its ligands. The membrane preparations exhibit a Kd of 0.39 nM for [125I]-WKYMVm. With 5 μg/well FPR1 Membrane Prep and 0.5 nM [125I]-WKYMVm, a greater than 20-fold signal-to-background is…
Chemicon's V1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of V1A interactions with its ligands. The membrane preparations exhibit a Kd of 55 pM for [125I]-vasopressin antagonist ([125I]-phenylacetyl-D-Tyr(Me)-Phe-Gln-Asn-Arg -Pro-Arg-Tyr-NH2). With 10 µg/well V1A Membr…
The MegaMan human transcriptome library is a collection of cDNA created using mRNA from 66 diverse sources, including 32 from human tissues and 34 from human cancer cell lines. This human transcriptome library is ideal for cloning both well-characterized genes and transcripts previously identified by expressed sequence tag (EST) studies. Largest comprehensive collection of the human transcriptome in a single tube Enriched…
The MegaMan human transcriptome library is a collection of cDNA created using mRNA from 66 diverse sources, including 32 from human tissues and 34 from human cancer cell lines. This human transcriptome library is ideal for cloning both well-characterized genes and transcripts previously identified by expressed sequence tag (EST) studies. Largest comprehensive collection of the human transcriptome in a single tube Enriched…
Bio S&T is the only commercial provider of highly purified human tRNAs. RNA is most widely used as the primer of retroviral reverse transcriptases. The 3’ end of the tRNA binds to the complementary binding site on the viral RNA therefore initiating reverse transcription. 1. tRNALys3 is the primer of HIV-I, HIV-II, SIV and FIV. 2. tRNALys1,2 are the primers of BLV, HTLV-I and II, SSV, FLV, VV, CAEV, MPMV, SMR and EIAV. 3. tRNAT…
May be used in a variety of laboratory assays Flat bottom strips are designed to easily break apart and may be used in an style strip holder that allows each individual well to be positioned back into the plate once broken High binding surface binds medium (>10kD) and large biomolecules that possess ionic groups and/or hydrophobic regions. Binding capacity is approximately 400 to 500ng IgG/cm2. Ideal for immunoassays Reco…
Activation of p38 MAP kinase is measured by an increase in fluorescence in the nucleus of celsl. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-p38 with a FITC emission filter. The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be appli…
(This is the suggested replacement for 3851)Excellent signal dynamic range due to low background fluorescence and enhanced signal intensitySuperior flatness -minimizes variability within a plate and between platesFlat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interac…
(This is the suggested replacement for 3852)Excellent signal dynamic range due to low background fluorescence and enhanced signal intensitySuperior flatness -minimizes variability within a plate and between platesFlat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interac…
Round well design with a solid black flat bottom 2.8mm distance between well bottoms to plate bottom Validated by Molecular Devices for the FLIPR reader. Outer wells aligned for proper viewing with FLIPR Ideal for miniaturized 384 well fluorescent homogeneous assays Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Suitable primarily for the…
No logo or lettering for improved heat sealing and ease of liquid handling Low base designed for optional bottom reader sensitivity Excellent signal dynamic range due to low background fluorescence and enhanced signal intensity Superior flatness -minimizes variability within a plate and between plates Flat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™ Not treated (or medium bi…
Cap Translucent flasks are supplied with a PFA screw cap. Screw caps provide hermetic seals and help reduce the possibility of outside contamination. The product is manufactured to Class A tolerances as defined by ISO 1042 and flasks have individually calibrated graduation lines. They withstand high temperatures and are resistant to a variety of aqueous solutions and organic solvents. These flasks may be autoclaved to 121ºC wi…
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…
(This is the suggested replacement for 3711) Ideally suited for fluorescent assays using either top or bottom detection plate readers Recommended working volume of up to 20 to 80 µL Conforms to standard microplate footprint and dimensions Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large mol…
(This is the suggested replacement for 3706)25 per Bag, without Lid, NonsterileIdeally suited for luminescent assays using either top or bottom detection plate readers Recommended working volume of up to 20 to 80 µL Conforms to standard microplate footprint and dimensions Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for th…
Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large molecules, such as antibodies, that have large hydrophobic regions that can interact with the surface Binding capacity of approximately 250 ng of mouse IgG/cm2 Certification results for each lot are made available upon request by contacting l…
EIA/RIA plates are tested on lot-to-lot basis and the certification results for each lot are made available upon request by contacting local Corning Life Sciences office High binding surface binds medium (>10kD) and large biomolecules that possess ionic groups and/or hydrophobic regions Binding capacity of approximately 500ng of mouse IgG/cm2 Easy Wash™ design (round to narrowed flat well bottom) for improved washing in i…
Has low background fluorescence and minimize light scattering Designed to reduce well-to-well crosstalk Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large molecules, such as antibodies, that have large hydrophobic regions that can interact with the surface Untreated microplates have a bindin…
High binding surface binds medium (>10kD) and large biomolecules that possess ionic groups and/or hydrophobic regions Binding capacity is approximately 400 to 500ng IgG/cm2 Ideal for immunoassays Without lids, lids supplied separately Bottoms are 60% thinner than conventional polystyrene plates, resulting in lower background fluorescent and enabling readings down to 340nm Designed to reduce well-to-well crosstalk during…
A cell-permeable (thienothienyl)amino-acetamide compound that displays anti-inflammatory properties. Acts as a potent, reversible, ATP-competitive, and highly selective inhibitor of IKK-2 (IC50 ~3-12 µM for IKK-2 homodimer, IKK-1/IKK-2 heterodimer, and IKK-2). Its specificity has been confirmed using a panel of 31 other kinases, including IKK isoforms IKK-1, IKK-i, and TBK-1 (IC50 > 200 µM). Shown to specifically block NF-kB-d…
ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency. Suitable fo…
The VisiTech laser engine combines the tightly collimated beams from multiple lasers into a single collinear beam. This module houses up to six solid state lasers with their associated controller systems. An optional fibre input port for combining externally generated laser lines is available. The AOTF module may also be integrated with this unit to permit computer control of laser line selection, individual line intensity a…
Has low background fluorescence and minimize light scattering Designed to reduce well-to-well crosstalk Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large molecules, such as antibodies, that have large hydrophobic regions that can interact with the surface Untreated microplates have a bindin…
