The Phusion™ Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with Quick T4 DNA Ligase. The resulting…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Kit contains all the necessary reagents for performing 50 amplifications in 50 μl volume or 125 amplifications in 20 μl volume. The kit also contains control template (λ DNA) and two primer sets (1.3 kb and 10 kb) for performing 2…
DyNAzyme™ II PCR Master Mix is available as a 2x mix containing recombinant DyNAzyme II DNA Polymerase (isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus), nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.
DyNAzyme™ I DNA Polymerase Kit contains DyNAzyme™ I DNA Polymerase, buffers, ready-to-use DNA size standard (λ DNA Hind III and øX174 DNA Hae III digest) and nucleotides for 100 amplifications in 50 μl volume. The kit also contains control template and primers for 40 control reactions.
RobusT™ I RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT I RT-PCR Kit is based on AMV Reverse Transcriptase (AMV RT) in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA amplificat…
RobusT™ II RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT II RT-PCR Kit is based on M-MuLV Reverse Transcriptase RNase H- in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA ampli…
Finnzymes' DyNAmo™ Flash Probe qPCR Kit allows enhanced performance for probe-based qPCR. Optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillar…
The i-control microplate reader software is a user-friendly interface for control of Tecan’s Infinite® series of microplate readers, enabling straightforward definition of workflows for various applications.
Phenogel™ Gel Permeation Chromatography Columns for size exclusion are available in seven different pore sizes ranging from 50 Å to 10 6 Å, and a linear bed configuration. Pore size distribution and pore volume are closely controlled parameters in the manufacturing process, accounting for high resolution, tight linear calibration curves and excellent column-to-column reproducibility.Phenogel GPC columns offer high cross-linkag…
Empore Filter Aid 400 has been shown to improve flow rates and recoveries for separation and filtration of water samples containing suspended solids. When placed on top of the Empore™ Extraction Disk at a depth of about 1 cm, Empore™ Filter Aid 400 acts as depth filter to inhibit the migration of suspended solids to the surface of the disk. This reduces extraction time and improves rates and recovery of analytes.
The AxyPrep™-96 Plasmid Purification Kit represents a true high-throughput system for the purification of plasmid or cosmid DNA in a 96-well format. The AxyPrep™-96 Plasmid Purification Kit is designed to produce up to 20 mg of highly purified plasmid or cosmid DNA (per well) from up to 1.3 ml of bacterial culture in 96-well format. With the AxyPrep™-96 Plasmid Purification Kit two plates rapidly and efficiently process the…
AxyPrep™ DNA Gel Extraction Kit employs optimized reagents in combination with a convenient spin column to purify DNA fragments from either TAE or TBE agarose gels (regular and low-melt). Each AxyPrep column in the AxyPrep DNA Gel Extraction Kit will bind up to 8 µg of DNA. DNA fragments in a size range of 75 bp up to 10 kb can be efficiently recovered with the AxyPrep DNA Gel Extraction Kit. Depending upon the length of the…
Fast Quality Checks for Bioethanol TestingSpecially designed for fast and accurate bioethanol analysis; improve method efficiency 800%Meet ASTM D5501 requirements - resolve methanol and ethanol from all other denaturant peaksGreat resolution of fusel alcoholsAllows for quick bake out between runs to eliminate contaminantsR&D 100 award-winning Zebron ZB-Bioethanol columns were designed to accelerate the bioethanol certification…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Exiqon miRCURY LNA™ microRNA Knockdown Probes High-affinity miRCURY LNA microRNA Knockdown Probesfor specific in vitro knockdown of microRNAs. Featuring minimal cytotoxicity and high biological stability. Key features of miRCURY LNA™ microRNA Knockdown Probes include:• Effective and sequence-specific antisense inhibition of microRNAs • Probes available for all known microRNAs • Superior biological stability • Minimal cytotoxi…
This ToxReporter Cell Line is used in conjunction with the ToxReporter ELISA kit (cat# ETR-201K) for the measurement of ToxReporter protein secreted into cell culture media as a consequence of exposure to a potential toxin. The cell line has been designed to respond to stimuli that induce cellular stress response pathways. Examples of stressful stimuli include compounds in drug discovery and organic or inorganic molecules pote…
LifeXtend™ conjugate stabilizer/diluent is a proprietary multi-component reagent system that stabilizes antibody-HRP conjugates both at 4°C and at ambient temperature. By using LifeXtend™ HRP stabilizer/diluent in antibody conjugation the HRP conjugate can be stabilized during use and stored as pre-diluted, ready-to-use reagents thus eliminating waste and improving consistency from experiment to experiment. LifeXtend™ shows…
The human KCNQ4 gene encodes the pore-forming subunit of a voltage-gated potassium channel. KCNQ4 channels expressed in CNS neurons are therapeutic targets in epilepsy. Synonyms: Kv7.4 Host cell: CHO-K1, division-arrested Gene name: KCNQ4 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth characteris…
The KCNH2 gene encodes the pore-forming subunit of a voltage-gated potassium channel. HERG channels expressed in cardiac muscle cells are safety targets; hERG inhibition has been linked to drug induced prolongation of the QT interval and torsade de pointes (a potentially lethal cardiac arrhythmia). Synonyms: Kv11.1 Host cell: CHO-K1, division-arrested Gene name: KCNH2 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Thr…
The Orbit LS low speed shaker is a high quality, general purpose shaker with economical analog control. Styled after the other models in the line, the Orbit LS features a streamlined, compact design with easy-access control panel. Electronic controls are utilized for setting speed and time. The knobs used for selecting parameters turn easily and are clearly marked with corresponding values. A large orbit and low speed make the…
The Labnet Economy Rockers, ProBlot 25 and 25XL, are basic rocking platforms that have been designed for a long life and trouble-free service. Rocker speed is adjustable to provide the gentle motion required for staining fragile gels as well as the more vigorous action necessary for washing blots. The rocking movement is fixed at ±7º. The platform surfaces are covered with nonslip rubber pads to prevent objects from moving dur…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
