Five Essential Neuroscience Imaging Applications

1. Correlation of Two-Photon In Vivo Imaging and FIB-SEM Microscopy
This white paper demonstrates that by using focused ion beam milling and crossbeam imaging, it is possible to observe the same dendrites at 7 nm isotropic resolution, which were formerly imaged over weeks with a NLO-system.

2.Development of 96/384-Well Kinetic Neurite Outgrowth/Stabilization Assays in Human iPSC-Derived Neurons Using Long Term Live Cell Imaging
This poster describes a novel in vitro assays for quantifying neurite parameters (length, branching) kinetically in human iPSC-derived neurons, in mono-culture and primary cryopreserved neurons and astrocytes in co-culture.

3. Visualization and Characterization of Autophagosomes in Neuronal Cells Using Enhanced Imaging Techniques This poster presents autophagy experiments using human induced pluripotent stem cell (iPSC)-derived human neurons or PC-12 cells. Results demonstrate the utility of the system and user-customizable analysis to rapidly test for multiple cell toxicity events in each well of a 384-well plate.

4. A High Throughput Drug Discovery for Intracellular Neurodegenerative Disease-Associated Targets using the LI-COR® Odyssey® Near Infrared Imaging System
Intracellular abnormal tau accumulation has been indicated in Alzheimer's disease (AD) progression. The LI-COR® Odyssey® scanner has been used to develop a novel, 96-well cell-based assay, suited for high throughput detection of changes in tau protein levels.

5. Identification and Spatial Localization of Proteins from Mouse Brain Tumor using a Combination of MALDI Imaging and LC-MALDI
This poster demonstrates the use of a LC-MALDI workflow to study treated and untreated tumors in mouse brain, to achieve spatial localization and protein identification.